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Literature summary for 1.14.14.16 extracted from

  • Kominami, S.; Ochi, H.; Kobayashi, Y.; Takemori, S.
    Studies on the steroid hydroxylation system in adrenal cortex microsomes (1980), J. Biol. Chem., 255, 3386-3394.
    View publication on PubMed
Search for more literature for 1.14.14.16

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 beef
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Storage Stability

Storage Stability Organism
0°C, 50 mM Tris, pH 7.2, 20% glycerol, 0.15% emulgen, 0.1 mM dithiothreitol, 0.1 mM EDTA, several weeks spectroscopically stable Bos taurus
25°C, 50 mM Tris, pH 7.2, 20% glycerol, 0.15% emulgen, 0.1 mM dithiothreitol, 0.1 mM EDTA, several hours spectroscopically stable, without Emulgen 50% precipitation after 30 min Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxyprogesterone + NADPH + O2
-
 Bos taurus 11-deoxycortisol + NADP+ + H2O
-
 ?
progesterone + NADPH + O2
-
 Bos taurus deoxycorticosterone + NADP+ + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.172
-
 progesterone
-
 Bos taurus
0.322
-
 17a-hydroxyprogesterone
-
 Bos taurus