Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.11 extracted from

  • Qaed, A.A.; Lin, H.; Tang, D.F.; Wu, Z.L.
    Rational design of styrene monooxygenase mutants with altered substrate preference (2011), Biotechnol. Lett., 33, 611-616.
    View publication on PubMed
Search for more literature for 1.14.14.11

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes in Escherichia coli Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
L44A mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene Pseudomonas putida
L45A mutation leads to a change of substrate preference. The mutant enzyme exhibits a remarkably reversed substrate preference compared to the wild type, displaying increased reactivity with an increase in substrate size. The activity of L45A is about 5% of that of the wild type towards styrene, but reaches about 30 and about 90% when the substrate is changed to 2-methylstyrene and 2-ethylstyrene, respectively Pseudomonas putida
N46A mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene Pseudomonas putida
R43A mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
styrene + FADH2 + O2 Pseudomonas putida
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?
styrene + FADH2 + O2 Pseudomonas putida LQ26
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida O33471
-
-
Pseudomonas putida LQ26 O33471
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ethylstyrene + FADH2 + O2 about 30% of the activity with styrene Pseudomonas putida (S)-2-ethyl-2-phenyloxirane + FAD + H2O
-
 ?
2-ethylstyrene + FADH2 + O2 about 30% of the activity with styrene Pseudomonas putida LQ26 (S)-2-ethyl-2-phenyloxirane + FAD + H2O
-
 ?
2-methylstyrene + FADH2 + O2 about 70% of the activity with styrene Pseudomonas putida (S)-2-methyl-2-phenyloxirane + FAD + H2O
-
 ?
2-methylstyrene + FADH2 + O2 about 70% of the activity with styrene Pseudomonas putida LQ26 (S)-2-methyl-2-phenyloxirane + FAD + H2O
-
 ?
styrene + FADH2 + O2
-
 Pseudomonas putida (S)-2-phenyloxirane + FAD + H2O
-
 ?
styrene + FADH2 + O2
-
 Pseudomonas putida LQ26 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Synonyms

Synonyms Comment Organism
StyA
-
 Pseudomonas putida