Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | a salt concentration of 162 mM results in half of the maximum inhibitory effect | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
styrene + FADH2 + O2 | mechanism: molecular oxygen first reacts with NSMOA(FADred) to yield an FAD C(4a)-peroxide intermediate. This species is nonfluorescent and has an absorbance maximum of 382 nm. Styrene then reacts with the peroxide intermediate to yield a fluorescent intermediate (FAD C(4a)-hydroxide) with an absorbance maximum of 368 nm | Pseudomonas putida | (S)-2-phenyloxirane + FAD + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NSMOA | styrene monooxygenase (SMO) is a two-component flavoenzyme composed of an NADH-specific flavin reductase (SMOB) and FAD-specific styrene epoxidase (NSMOA) | Pseudomonas putida |
SMO | styrene monooxygenase (SMO) is a two-component flavoenzyme composed of an NADH-specific flavin reductase (SMOB) and FAD-specific styrene epoxidase (NSMOA) | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | NSMOA binds tightly to reduced FAD. FAD C(4a)-peroxide is the oxygen atom donor | Pseudomonas putida |