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Literature summary for 1.14.14.1 extracted from

  • Haarmann, T.; Ortel, I.; Tudzynski, P.; Keller, U.
    Identification of the cytochrome P450 monooxygenase that bridges the clavine and ergoline alkaloid pathways (2006), Chembiochem, 7, 645-652.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cloA, localization in the ergot alkaloid gene cluster, DNA and amino acid sequence determination and analysis, complementation of enzyme-deficient mutant DELTAP450-1 Claviceps purpurea

Protein Variants

Protein Variants Comment Organism
additional information construction of a disrution-inactivated, enzyme-deficient mutant DELTAP450-1, that accumulates agroclavine, elymoclavine, and chanoclavine, but not ergopeptines, feeding experiments Claviceps purpurea

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a heme iron, the enzyme is a cytochrome P450 monooxygenase Claviceps purpurea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58200
-
x * 58200, amino acid sequence calculation Claviceps purpurea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 Claviceps purpurea
-
paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O the product is the precursor for D-lysergic acid ?
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 Claviceps purpurea P1
-
paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O the product is the precursor for D-lysergic acid ?
additional information Claviceps purpurea the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster ?
-
?
additional information Claviceps purpurea P1 the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster ?
-
?

Organism

Organism UniProt Comment Textmining
Claviceps purpurea
-
gene cloA
-
Claviceps purpurea P1
-
gene cloA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2
-
Claviceps purpurea paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2
-
Claviceps purpurea paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O the product is the precursor for D-lysergic acid ?
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2
-
Claviceps purpurea P1 paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2
-
Claviceps purpurea P1 paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O the product is the precursor for D-lysergic acid ?
additional information the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster Claviceps purpurea ?
-
?
additional information the enzyme possibly also catalyzes the oxidation of agroclavine, overview Claviceps purpurea ?
-
?
additional information the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster Claviceps purpurea P1 ?
-
?
additional information the enzyme possibly also catalyzes the oxidation of agroclavine, overview Claviceps purpurea P1 ?
-
?

Subunits

Subunits Comment Organism
? x * 58200, amino acid sequence calculation Claviceps purpurea

Synonyms

Synonyms Comment Organism
clavine oxidase
-
Claviceps purpurea
CLOA
-
Claviceps purpurea
cytochrome P450 monooxygenase
-
Claviceps purpurea

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Claviceps purpurea