Cloned (Comment) | Organism |
---|---|
gene cloA, localization in the ergot alkaloid gene cluster, DNA and amino acid sequence determination and analysis, complementation of enzyme-deficient mutant DELTAP450-1 | Claviceps purpurea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a disrution-inactivated, enzyme-deficient mutant DELTAP450-1, that accumulates agroclavine, elymoclavine, and chanoclavine, but not ergopeptines, feeding experiments | Claviceps purpurea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a heme iron, the enzyme is a cytochrome P450 monooxygenase | Claviceps purpurea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58200 | - |
x * 58200, amino acid sequence calculation | Claviceps purpurea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 | Claviceps purpurea | - |
paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | the product is the precursor for D-lysergic acid | ? | |
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 | Claviceps purpurea P1 | - |
paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | the product is the precursor for D-lysergic acid | ? | |
additional information | Claviceps purpurea | the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster | ? | - |
? | |
additional information | Claviceps purpurea P1 | the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Claviceps purpurea | - |
gene cloA | - |
Claviceps purpurea P1 | - |
gene cloA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Claviceps purpurea | paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Claviceps purpurea | paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | the product is the precursor for D-lysergic acid | ? | |
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Claviceps purpurea P1 | paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
elymoclavine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Claviceps purpurea P1 | paspalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | the product is the precursor for D-lysergic acid | ? | |
additional information | the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster | Claviceps purpurea | ? | - |
? | |
additional information | the enzyme possibly also catalyzes the oxidation of agroclavine, overview | Claviceps purpurea | ? | - |
? | |
additional information | the enzyme possibly also catalyzes the oxidation of agroclavine, overview, the enzyme bridges the clavine and ergoline alkaloid pathways, it is critical in the ergot alkaloid gene cluster | Claviceps purpurea P1 | ? | - |
? | |
additional information | the enzyme possibly also catalyzes the oxidation of agroclavine, overview | Claviceps purpurea P1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58200, amino acid sequence calculation | Claviceps purpurea |
Synonyms | Comment | Organism |
---|---|---|
clavine oxidase | - |
Claviceps purpurea |
CLOA | - |
Claviceps purpurea |
cytochrome P450 monooxygenase | - |
Claviceps purpurea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Claviceps purpurea |