Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
P440F | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
P440H | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
P440I | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
P440L | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
P440N | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
P440T | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
P440Y | higher subtrate variability, temperature optimum at 50C with range from 45-58C | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetone + NADPH + O2 | reaction is performed in presence of 2 U secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus and isopropanol to recover NADPH | Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? | |
rac-2-ethylcyclohexanone + NADPH + O2 | substrate is only accepted by mutants of phenylacetone monooxygenase, reaction is performed in presence of 2 U secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus and isopropanol to recover NADPH | Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Baeyer-Villiger monooxygenase | - |
Thermobifida fusca |
BVMO | - |
Thermobifida fusca |
PAMO | - |
Thermobifida fusca |
phenylacetone monooxygenase | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
- |
Thermobifida fusca |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 58 | at 58C 75% of enzyme activity remains, at 60C activity is nearly zero, at 45C enzyme is fully active | Thermobifida fusca |