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Literature summary for 1.14.13.92 extracted from

  • Fraaije, M.W.; Wu, J.; Heuts, D.P.; van Hellemond, E.W.; Spelberg, J.H.; Janssen, D.B.
    Discovery of a thermostable Baeyer-Villiger monooxygenase by genome mining (2005), Appl. Microbiol. Biotechnol., 66, 393-400.
    View publication on PubMed
Search for more literature for 1.14.13.92

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermobifida fusca

General Stability

General Stability Organism
PAMO that has been activated at 50°C can be stored for several days at room temperature without any loss in activity Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
 phenylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
0.26
-
 2-dodecanone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
0.36
-
 benzylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
0.83
-
 (2-methylphenyl)acetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
0.86
-
 methyl 4-tolylsulfide 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
2.2
-
 4-hydroxyacetophenone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
15
-
 bicyclohept-2-en-6-one 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 1 * 65000, SDS-PAGE, small amount of approx. 7% is present as a dimer Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PAMO Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-methylphenyl)acetone + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
2-dodecanone + NADPH + O2
-
 Thermobifida fusca ? + NADP+
-
 ?
4-hydroxyacetophenone + NADPH + O2
-
 Thermobifida fusca acetic acid 4-hydroxyphenyl ester + NADP+ + O2
-
 ?
benzylacetone + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + H2O
-
 ?
bicyclohept-2-en-6-one + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
ethionamide + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
methyl 4-tolylsulfide + NADPH + O2
-
 Thermobifida fusca ? + NADP+ + O2
-
 ?
phenylacetone + NADPH + H+ + O2
-
 Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE, small amount of approx. 7% is present as a dimer Thermobifida fusca

Synonyms

Synonyms Comment Organism
Baeyer-Villiger monooxygenase
-
 Thermobifida fusca
BVMO
-
 Thermobifida fusca
PAMO
-
 Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 4fold higher activity when compared with the activity at 25°C Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 50% loss of activity after 24 h and 48 h in the absence and presence of FAD, respectively Thermobifida fusca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 2-dodecanone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
0.34
-
 4-hydroxyacetophenone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.1
-
 bicyclohept-2-en-6-one 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.8
-
 benzylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
1.9
-
 phenylacetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
2
-
 (2-methylphenyl)acetone 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca
2.1
-
 methyl 4-tolylsulfide 30°C, pH 7.5, 0.1 mM NADPH Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermobifida fusca

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 80% of maximal activity Thermobifida fusca

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Thermobifida fusca
NADPH can not be replaced by NADH Thermobifida fusca