Literature summary for 1.14.13.9 extracted from

Winkler, D.; Beconi, M.; Toledo-Sherman, L.M.; Prime, M.; Ebneth, A.; Dominguez, C.; Munoz-Sanjuan, I.
Development of LC/MS/MS, high-throughput enzymatic and cellular assays for the characterization of compounds that inhibit kynurenine monooxygenase (KMO) (2013), J. Biomol. Screen., 18, 879-889.

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Application

Application	Comment	Organism
analysis	comprehensive panel of biochemical and cell-based assays that use liquid chromatography/tandem mass spectrometry to quantify unlabeled kynurenine and 3-hydroxykynurenine and application to measure kynurenine monooxygenase inhibition in cell and tissue extracts, as well as cellular assays	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
4-(3,4-dichlorophenyl)-4-oxobutanoic acid	desamino FCE 28833	Homo sapiens
Ro 61-8048	-	Homo sapiens
UPF 648	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	kynurenine	liver lysate, pH 7.4, 25°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
kynurenine + NADPH + O2	-	Homo sapiens	3-hydroxy-kynurenine + NADP+ + H2O	-	?

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0000003	-	pH 7.4, 25°C	Homo sapiens	UPF 648
0.000025	-	pH 7.4, 25°C	Homo sapiens	4-(3,4-dichlorophenyl)-4-oxobutanoic acid
0.000035	-	pH 7.4, 25°C	Homo sapiens	Ro 61-8048

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Release 2023.1 (January 2023)