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Literature summary for 1.14.13.84 extracted from
- Conversion of 4-hydroxyacetophenone into 4-phenyl acetate by a flavin adenine dinucleotide-containing Baeyer-Villiger-type monooxygenase (2000), J. Bacteriol., 182, 6565-6569.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0175 | - |
NADPH | pH 8, 30°C, 4-hydroxyacetophenone as substrate | Pseudomonas putida |  |
| 0.023 | - |
4-hydroxypropiophenone | pH 8, 30°C | Pseudomonas putida | |
| 0.047 | - |
4-hydroxyacetophenone | pH 8, 30°C | Pseudomonas putida | |
| 0.384 | - |
acetophenone | pH 8, 30°C | Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Pseudomonas putida |
| 71000 | - |
1 * 71000, SDS-PAGE | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyacetophenone + NADPH + H+ + O2 | Pseudomonas putida | involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
| 4-hydroxyacetophenone + NADPH + H+ + O2 | Pseudomonas putida JD1 | involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida JD1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 98fold | Pseudomonas putida |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (4-hydroxyphenyl)ethan-1-one + NADPH + H+ + O2 = 4-hydroxyphenyl acetate + NADP+ + H2O | contains FAD, the enzyme from Pseudomonas fluorescens ACB catalyses the conversion of a wide range of acetophenone derivatives, highest activity occurs with compounds bearing an electron-donating substituent at the para position of the aromatic ring, in the absence of substrate, the enzyme can act as an NADPH oxidase (EC 1.6.3.1) | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
pH 8, 30°C | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyacetophenone + NADPH + H+ + O2 | consumption of one molecule of oxygen and oxidation of one molecule of NADPH per substrate molecule, specific for NADPH as electron donor, requirement for O2 | Pseudomonas putida | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
| 4-hydroxyacetophenone + NADPH + H+ + O2 | involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme | Pseudomonas putida | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
| 4-hydroxyacetophenone + NADPH + H+ + O2 | consumption of one molecule of oxygen and oxidation of one molecule of NADPH per substrate molecule, specific for NADPH as electron donor, requirement for O2 | Pseudomonas putida JD1 | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
| 4-hydroxyacetophenone + NADPH + H+ + O2 | involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme | Pseudomonas putida JD1 | 4-hydroxyphenyl acetate + NADP+ + H2O | - |
? | |
| 4-hydroxypropiophenone + NADPH + H+ + O2 | specific for NADPH as electron donor, requirement for O2 | Pseudomonas putida | 4-hydroxyphenyl propionate + NADP+ + H2O | - |
? | |
| 4-hydroxypropiophenone + NADPH + H+ + O2 | specific for NADPH as electron donor, requirement for O2 | Pseudomonas putida JD1 | 4-hydroxyphenyl propionate + NADP+ + H2O | - |
? | |
| acetophenone + NADPH + H+ + O2 | specific for NADPH as electron donor, requirement for O2 | Pseudomonas putida | phenyl acetate + NADP+ + H2O | - |
? | |
| acetophenone + NADPH + H+ + O2 | specific for NADPH as electron donor, requirement for O2 | Pseudomonas putida JD1 | phenyl acetate + NADP+ + H2O | - |
? | |
| additional information | not: benzophenone, cyclohexylacetone, NADH | Pseudomonas putida | ? | - |
? | |
| additional information | not: benzophenone, cyclohexylacetone, NADH | Pseudomonas putida JD1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 71000, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylketone monooxygenase | - |
Pseudomonas putida |
| HAPMO | - |
Pseudomonas putida |
| More | Baeyer-Villiger-type monooxygenase | Pseudomonas putida |
| oxygenase, 4-hydroxyacetophenone mono- | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
Tris-HCl buffer | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains one molecule of FAD per enzyme molecule, FAD is reduced specifically by NADPH and not by NADH | Pseudomonas putida | |
| additional information | not: NADH | Pseudomonas putida | |
| NADPH | uses NADPH as cofactor | Pseudomonas putida | |
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