Cloned (Comment) | Organism |
---|---|
genes vanA and vanB, DNA and amino acid sequence determination and analysis, functional coexpression of genes vanA and vanB in Escherichia coli K12 strain WV1181 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the reaction of the recombinant enzyme is one of the limiting steps of the prospective bioconversion from lignin-derived compounds to valuable products in recombinant Escherichia coli | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillic acid + NAD(P)H + H+ + O2 | Pseudomonas putida | functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway, formaldehyde is further converted to formate by glutathione-dependent formaldehyde dehydrogenase, encoded by gene frmA | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | procatechuic acid is 3,4-dihydroxybenzoate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.003 | 0.88 | recombinant whole cell activities with both cofactors NADPH or NADH | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillic acid + NAD(P)H + H+ + O2 | - |
Pseudomonas putida | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | procatechuic acid is 3,4-dihydroxybenzoate | ? | |
vanillic acid + NAD(P)H + H+ + O2 | functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway, formaldehyde is further converted to formate by glutathione-dependent formaldehyde dehydrogenase, encoded by gene frmA | Pseudomonas putida | protocatechuic acid + NAD(P)+ + H2O + formaldehyde | procatechuic acid is 3,4-dihydroxybenzoate | ? |
Synonyms | Comment | Organism |
---|---|---|
vanillate-O-demethylase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Pseudomonas putida |