Crystallization (Comment) | Organism |
---|---|
mutant E158A/E159A, ligand-free or in complex with NADP+, microbatch technique at 4°C by mixing equal volumes of 8 mg of protein/ml in 25 mM Tris-HCl, pH 8.0, 250 mM NaCl, 1 mM NADP+, and of crystallization solution containing PEG 4000 20% w/v in 0.1 M Na/HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, molecular replacement | Methylophaga aminisulfidivorans |
Protein Variants | Comment | Organism |
---|---|---|
E158A/E159A | the mutant shows similar activity with trimethylamine compared to the wild-type enzyme | Methylophaga aminisulfidivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADPH | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
0.066 | - |
Methimazole | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
0.073 | - |
trimethylamine | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
0.09 | - |
indole | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
0.13 | - |
Nicotine | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
0.232 | - |
N,N-Dimethylaniline | pH 8.5, 25°C | Methylophaga aminisulfidivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Methylophaga aminisulfidivorans | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Methylophaga aminisulfidivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylaniline + NADPH + H+ + O2 | Methylophaga aminisulfidivorans | - |
N,N-dimethylaniline N-oxide + NADP+ + H2O | - |
? | |
N,N-dimethylaniline + NADPH + H+ + O2 | Methylophaga aminisulfidivorans SK1 | - |
N,N-dimethylaniline N-oxide + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophaga aminisulfidivorans | - |
- |
- |
Methylophaga aminisulfidivorans SK1 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N,N-dimethylaniline + NADPH + H+ + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O | catalytic cycle, reaction mechanism via C4a-hydroperoxyflavin intermediate and structure-function relationship, overview | Methylophaga aminisulfidivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans | indoxyl + NADP+ + H2O | - |
? | |
indole + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans SK1 | indoxyl + NADP+ + H2O | - |
? | |
methimazole + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans | ? | - |
? | |
methimazole + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans SK1 | ? | - |
? | |
additional information | substrate specificity, the ability to stabilize the hydroperoxyflavin intermediate in substrate oxygenation is crucial involving NADP(H), overview | Methylophaga aminisulfidivorans | ? | - |
? | |
additional information | substrate specificity, the ability to stabilize the hydroperoxyflavin intermediate in substrate oxygenation is crucial involving NADP(H), overview | Methylophaga aminisulfidivorans SK1 | ? | - |
? | |
N,N-dimethylaniline + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans | N,N-dimethylaniline N-oxide + NADP+ + H2O | - |
? | |
N,N-dimethylaniline + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans SK1 | N,N-dimethylaniline N-oxide + NADP+ + H2O | - |
? | |
nicotine + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans | nicotine N-oxide + NADP+ + H2O | - |
? | |
trimethylamine + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans | trimethylamine N-oxide + NADP+ + H2O | - |
? | |
trimethylamine + NADPH + H+ + O2 | - |
Methylophaga aminisulfidivorans SK1 | trimethylamine N-oxide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Methylophaga aminisulfidivorans |
Synonyms | Comment | Organism |
---|---|---|
FMO | - |
Methylophaga aminisulfidivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methylophaga aminisulfidivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
NADPH | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
0.7 | - |
indole | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
1 | - |
Methimazole | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
1.8 | - |
N,N-Dimethylaniline | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
3 | - |
Nicotine | pH 8.5, 25°C | Methylophaga aminisulfidivorans | |
6.1 | - |
trimethylamine | pH 8.5, 25°C | Methylophaga aminisulfidivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Methylophaga aminisulfidivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | binding structure, one molecule of noncovalently tightly bound FAD per enzyme monomer, overview | Methylophaga aminisulfidivorans | |
NADPH | binding structure, the adjacent ribose of NADP+ is an integral part of the catalytic site being actively engaged in the stabilization of the oxygenating intermediate, overview | Methylophaga aminisulfidivorans |