Cloned (Comment) | Organism |
---|---|
expression of His-tagged EtaA in Escherichia coli strain DH5alpha | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Mycobacterium tuberculosis | |
0.131 | - |
thiacetazone | recombinant EtaA, pH 9.0, 37°C, thiacetazone sulfinic acid formation | Mycobacterium tuberculosis | |
0.147 | - |
thiacetazone | recombinant EtaA, pH 9.0, 37°C, thiacetazone carbodiimide formation | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethionamide + NADPH + O2 + H+ | Mycobacterium tuberculosis | bioactivation by EtaA | 2-ethyl-N-hydroxypyridine-4-carbothioamide + NADP+ + H2O | - |
? | |
ethionamide + NADPH + O2 + H+ | Homo sapiens | bioactivation by isozymes FMO1 and FMO3 | 2-ethyl-N-hydroxypyridine-4-carbothioamide + NADP+ + H2O | - |
? | |
thiacetazone + 2 NADPH + 2 H+ + 2 O2 | Mycobacterium tuberculosis | bioactivation by EtaA | thiacetazone carbodiimide + 2 NADP+ + 2 H2O | - |
? | |
thiacetazone + 2 NADPH + 2 H+ + 2 O2 | Homo sapiens | bioactivation by isozymes FMO1 and FMO3, two-step process | thiacetazone carbodiimide + 2 NADP+ + 2 H2O | - |
? | |
thiacetazone + 2 NADPH + 2 O2 | Mycobacterium tuberculosis | bioactivation by EtaA | (E)-{(2E)-[4-(acetylamino)benzylidene]hydrazinylidene}(amino)methanesulfinic acid + 2 NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes FMO1 and FMO3 | - |
Mycobacterium tuberculosis | - |
gene etaA or RV3854c | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged EtaA from Escherichia coli strain DH5alpha by nickel affinity chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
additional information | - |
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethionamide + NADPH + O2 + H+ | bioactivation by EtaA | Mycobacterium tuberculosis | 2-ethyl-N-hydroxypyridine-4-carbothioamide + NADP+ + H2O | - |
? | |
ethionamide + NADPH + O2 + H+ | bioactivation by isozymes FMO1 and FMO3 | Homo sapiens | 2-ethyl-N-hydroxypyridine-4-carbothioamide + NADP+ + H2O | - |
? | |
ethionamide + NADPH + O2 + H+ | a thioamide-containing second line antitubercular prodrug | Homo sapiens | 2-ethyl-N-hydroxypyridine-4-carbothioamide + NADP+ + H2O | - |
? | |
ethionamide + NADPH + O2 + H+ | a thioamide-containing second line antitubercular prodrug | Mycobacterium tuberculosis | 2-ethyl-N-hydroxypyridine-4-carbothioamide + NADP+ + H2O | - |
? | |
thiacetazone + 2 NADPH + 2 H+ + 2 O2 | bioactivation by EtaA | Mycobacterium tuberculosis | thiacetazone carbodiimide + 2 NADP+ + 2 H2O | - |
? | |
thiacetazone + 2 NADPH + 2 H+ + 2 O2 | bioactivation by isozymes FMO1 and FMO3, two-step process | Homo sapiens | thiacetazone carbodiimide + 2 NADP+ + 2 H2O | - |
? | |
thiacetazone + 2 NADPH + 2 H+ + 2 O2 | a thiourea-containing second line antitubercular prodrug | Homo sapiens | thiacetazone carbodiimide + 2 NADP+ + 2 H2O | - |
? | |
thiacetazone + 2 NADPH + 2 H+ + 2 O2 | a thiourea-containing second line antitubercular prodrug | Mycobacterium tuberculosis | thiacetazone carbodiimide + 2 NADP+ + 2 H2O | - |
? | |
thiacetazone + 2 NADPH + 2 O2 | bioactivation by EtaA | Mycobacterium tuberculosis | (E)-{(2E)-[4-(acetylamino)benzylidene]hydrazinylidene}(amino)methanesulfinic acid + 2 NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EtaA | - |
Mycobacterium tuberculosis |
FMO | - |
Homo sapiens |
FMO | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
thiacetazone | recombinant EtaA, pH 9.0, 37°C, thiacetazone carbodiimide formation | Mycobacterium tuberculosis | |
0.085 | - |
thiacetazone | recombinant EtaA, pH 9.0, 37°C, thiacetazone sulfinic acid formation | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Homo sapiens |
9 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | pH profile | Mycobacterium tuberculosis |
6.5 | 10 | isozyme FMO1, pH profile | Homo sapiens |
7.5 | 10 | isozyme FMO3, pH profile | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
FAD | - |
Mycobacterium tuberculosis | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Mycobacterium tuberculosis |