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Literature summary for 1.14.13.8 extracted from

  • Prough, R.A.; Freeman, P.C.; Hines, R.N.
    The oxidation of hydrazine derivatives catalyzed by the purified liver microsomal FAD-containing monooxygenase (1981), J. Biol. Chem., 256, 4178-4184.
    View publication on PubMed
Search for more literature for 1.14.13.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 N,N-Dimethylaniline
-
 Sus scrofa
0.03
-
 N-Aminopiperidine
-
 Sus scrofa
0.08
-
 1-Methyl-1-phenylhydrazine
-
 Sus scrofa
0.1
-
 N-aminopyrrolidine
-
 Sus scrofa
0.17
-
 N-Aminohomopiperidine
-
 Sus scrofa
0.38
-
 1,1-Dimethylhydrazine
-
 Sus scrofa
0.61
-
 N-aminomorpholine
-
 Sus scrofa
2
-
 1-methyl-2-benzylhydrazine
-
 Sus scrofa
3
-
 phenylhydrazine
-
 Sus scrofa
3.3
-
 beta-ethylphenylhydrazine
-
 Sus scrofa
5.7
-
 procarbazine
-
 Sus scrofa
6.9
-
 butylhydrazine
-
 Sus scrofa
7
-
 benzylhydrazine
-
 Sus scrofa
8.3
-
 isopropylhydrazine
-
 Sus scrofa
12
-
 1,2-dimethylhydrazine
-
 Sus scrofa
15
-
 n-propylhydrazine
-
 Sus scrofa
35
-
 methylhydrazine
-
 Sus scrofa
40
-
 ethylhydrazine
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,1-dimethylhydrazine + NADPH + O2 Rattus norvegicus possibly, and other 1,1-disubstituted hydrazines formaldehyde + CH3N2H3 + NADP+
-
 ?
1,1-dimethylhydrazine + NADPH + O2 Sus scrofa possibly, and other 1,1-disubstituted hydrazines formaldehyde + CH3N2H3 + NADP+
-
 ?
1,1-dimethylhydrazine + NADPH + O2 Mesocricetus auratus possibly, and other 1,1-disubstituted hydrazines formaldehyde + CH3N2H3 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-
liver
-
 Mesocricetus auratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1-dimethylhydrazine + NADPH + O2
-
 Rattus norvegicus formaldehyde + CH3N2H3 + NADP+
-
 ?
1,1-dimethylhydrazine + NADPH + O2
-
 Sus scrofa formaldehyde + CH3N2H3 + NADP+
-
 ?
1,1-dimethylhydrazine + NADPH + O2
-
 Mesocricetus auratus formaldehyde + CH3N2H3 + NADP+
-
 ?
1,1-dimethylhydrazine + NADPH + O2 possibly, and other 1,1-disubstituted hydrazines Rattus norvegicus formaldehyde + CH3N2H3 + NADP+
-
 ?
1,1-dimethylhydrazine + NADPH + O2 possibly, and other 1,1-disubstituted hydrazines Sus scrofa formaldehyde + CH3N2H3 + NADP+
-
 ?
1,1-dimethylhydrazine + NADPH + O2 possibly, and other 1,1-disubstituted hydrazines Mesocricetus auratus formaldehyde + CH3N2H3 + NADP+
-
 ?
1,2-dimethylhydrazine + NADPH + H+ + O2
-
 Sus scrofa 1,2-dimethylhydrazine N-oxide + NADP+ + H2O
-
 r
1,2-dimethylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
1,2-dimethylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
1,2-dimethylphenylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
1,2-dimethylphenylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
1-methyl-1-phenylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
1-methyl-1-phenylhydrazine + NADPH + O2
-
 Sus scrofa ?
-
 ?
1-methyl-1-phenylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
1-methyl-2-benzylhydrazine + NADPH + H+ + O2
-
 Sus scrofa 1-methyl-2-benzylhydrazine N-oxide + NADP+ + H2O
-
 r
1-methyl-2-benzylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
1-methyl-2-benzylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
benzylhydrazine + NADPH + H+ + O2
-
 Sus scrofa benzylhydrazine N-oxide + NADP+ + H2O
-
 r
benzylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
benzylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
beta-ethylphenylhydrazine + NADPH + H+ + O2
-
 Sus scrofa beta-ethylphenylhydrazine N-oxide + NADP+ + H2O
-
 r
beta-ethylphenylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
beta-ethylphenylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
butylhydrazine + NADPH + H+ + O2
-
 Sus scrofa butylhydrazine N-oxide + NADP+ + H2O
-
 r
butylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
butylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
ethylhydrazine + NADPH + H+ + O2
-
 Sus scrofa ethylhydrazine N-oxide + NADP+ + H2O
-
 r
isopropylhydrazine + NADPH + H+ + O2
-
 Sus scrofa isopropylhydrazine N-oxide + NADP+ + H2O
-
 r
isopropylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
isopropylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
N,N-dimethylaniline + NADPH + H+ + O2
-
 Sus scrofa N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 r
N,N-dimethylaniline + NADPH + O2
-
 Rattus norvegicus N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?
N,N-dimethylaniline + NADPH + O2
-
 Sus scrofa N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?
N,N-dimethylaniline + NADPH + O2
-
 Mesocricetus auratus N,N-dimethylaniline N-oxide + NADP+ + H2O
-
 ?
N-aminohomopiperidine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
N-aminohomopiperidine + NADPH + O2
-
 Sus scrofa ?
-
 ?
N-aminohomopiperidine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
N-aminomorpholine + NADPH + H+ + O2
-
 Sus scrofa N-aminomorpholine N-oxide + NADP+ + H2O
-
 r
N-aminomorpholine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
N-aminomorpholine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
N-aminopiperidine + NADPH + O2
-
 Rattus norvegicus tetrazene + NADP+ + H2O + ?
-
 ?
N-aminopiperidine + NADPH + O2
-
 Sus scrofa tetrazene + NADP+ + H2O + ?
-
 ?
N-aminopiperidine + NADPH + O2
-
 Mesocricetus auratus tetrazene + NADP+ + H2O + ?
-
 ?
N-aminopiperidine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
N-aminopiperidine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
N-aminopyrrolidine + NADPH + H+ + O2
-
 Sus scrofa N-aminopyrrolidine N-oxide + NADP+ + H2O
-
 r
N-aminopyrrolidone + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
N-aminopyrrolidone + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
n-propylhydrazine + NADPH + H+ + O2
-
 Sus scrofa N-propylhydrazine N-oxide + NADP+ + H2O
-
 r
phenylhydrazine + NADPH + H+ + O2
-
 Sus scrofa phenylhydrazine N-oxide + NADP+ + H2O
-
 r
phenylhydrazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
phenylhydrazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?
procarbazine + NADPH + H+ + O2
-
 Sus scrofa procarbazine N-oxide + NADP+ + H2O
-
 r
procarbazine + NADPH + O2
-
 Rattus norvegicus ?
-
 ?
procarbazine + NADPH + O2
-
 Mesocricetus auratus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 demethylation of 1,1-dimethylhydrazine Mesocricetus auratus
8.5
-
 hydrazine oxidation Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: about 40% of activity maximum, pH 9.0: about 95% of activity maximum Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Rattus norvegicus
FAD flavoprotein Sus scrofa
FAD flavoprotein Mesocricetus auratus
NADPH
-
 Rattus norvegicus
NADPH
-
 Sus scrofa
NADPH
-
 Mesocricetus auratus