Cloned (Comment) | Organism |
---|---|
oxygenase component PHO | Acinetobacter radioresistens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11600 | - |
(alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE | Acinetobacter radioresistens |
37800 | - |
(alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE | Acinetobacter radioresistens |
54000 | - |
(alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE | Acinetobacter radioresistens |
207000 | - |
oxygenase component PHO, gel filtration | Acinetobacter radioresistens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter radioresistens | - |
S13 | - |
Acinetobacter radioresistens S13 | - |
S13 | - |
Purification (Comment) | Organism |
---|---|
oxygenase component PHO | Acinetobacter radioresistens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dimethylphenol + NADH + H+ + O2 | 85% of the activity with phenol | Acinetobacter radioresistens | 1,2-dihydroxy-3,4-dimethylbenzene + NAD+ + H2O | - |
? | |
3,4-dimethylphenol + NADH + H+ + O2 | 85% of the activity with phenol | Acinetobacter radioresistens S13 | 1,2-dihydroxy-3,4-dimethylbenzene + NAD+ + H2O | - |
? | |
3-chlorophenol + NADH + H+ + O2 | 15% of the activity with phenol | Acinetobacter radioresistens | 4-chlorocatechol + NAD+ + H2O | - |
? | |
3-chlorophenol + NADH + H+ + O2 | 15% of the activity with phenol | Acinetobacter radioresistens S13 | 4-chlorocatechol + NAD+ + H2O | - |
? | |
4-chlorophenol + NADH + H+ + O2 | - |
Acinetobacter radioresistens | 1,2-dihydroxy-4-methylbenzene + NAD+ + H2O | - |
? | |
4-chlorophenol + NADH + H+ + O2 | - |
Acinetobacter radioresistens S13 | 1,2-dihydroxy-4-methylbenzene + NAD+ + H2O | - |
? | |
4-chlorophenol + NADH + H+ + O2 | 27% of the activity with phenol | Acinetobacter radioresistens | 4-chlorocatechol + NAD+ + H2O | - |
? | |
4-fluorophenol + NADH + H+ + O2 | - |
Acinetobacter radioresistens | 1,2-dihydroxy-4-fluorobenzene + NAD+ + H2O | - |
? | |
4-methylphenol + NADH + H+ + O2 | - |
Acinetobacter radioresistens | 1,2-dihydroxy-4-methylbenzene + NAD+ + H2O | - |
? | |
m-cresol + NADH + H+ + O2 | 60% of the activity with phenol | Acinetobacter radioresistens | 4-methylcatechol + NAD+ + H2O | - |
? | |
additional information | no PHO activity with p-hydroxybenzoic acid, m-hydroxybenzoic acid, 2,3-dinitrophenol, 3,4-dichlorophenol, 2,4,5-trichlorophenol, 2,2'-dihydroxybiphenyl and L-Tyr | Acinetobacter radioresistens | ? | - |
? | |
additional information | no PHO activity with p-hydroxybenzoic acid, m-hydroxybenzoic acid, 2,3-dinitrophenol, 3,4-dichlorophenol, 2,4,5-trichlorophenol, 2,2'-dihydroxybiphenyl and L-Tyr | Acinetobacter radioresistens S13 | ? | - |
? | |
o-cresol + NADH + H+ + O2 | 60% of the activity with phenol | Acinetobacter radioresistens | 3-methylcatechol + NAD+ + H2O | - |
? | |
p-cresol + NADH + H+ + O2 | 60% of the activity with phenol | Acinetobacter radioresistens | 4-methylcatechol + NAD+ + H2O | - |
? | |
phenol + NADH + H+ + O2 | - |
Acinetobacter radioresistens | catechol + NAD+ + H2O | - |
? | |
phenol + NADH + H+ + O2 | - |
Acinetobacter radioresistens S13 | catechol + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | (alphabetagamma)2, 2 * 54000 + 2 * 37800 + 2 * 11600, oxygenase component PHO, SDS-PAGE | Acinetobacter radioresistens |
Synonyms | Comment | Organism |
---|---|---|
phenol hydroxylase | multicomponent enzyme made up of three moieties: a reductase (PHR), and an oxygenase (PHO) and a regulative component | Acinetobacter radioresistens |
PHO | oxygenase component of the phenyl hydroxylase | Acinetobacter radioresistens |
PHR | reductase component of phenyl hydroxylase | Acinetobacter radioresistens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
and a second smaller peak at 32°C | Acinetobacter radioresistens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 37 | 20°C: about 70% of maximal activity, 24°C: maximal activity, 30°C: about 40% of maximal activity, 32°C: about 65% of maximal activity, 37°C: about 30% of maximal activity, oxygenase component PHO | Acinetobacter radioresistens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1980 | - |
NADH | oxygenase component PHO | Acinetobacter radioresistens | |
1980 | - |
phenol | oxygenase component PHO | Acinetobacter radioresistens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
oxygenase component PHO | Acinetobacter radioresistens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 45% of maximal activity, pH 8.5: about 30% of maximal activity, oxygenase component PHO | Acinetobacter radioresistens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Acinetobacter radioresistens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acinetobacter radioresistens | oxygenase component PHO | - |
6.7 |