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Literature summary for 1.14.13.6 extracted from

  • Ohta, Y.; Ribbons, D.W.
    Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC (1976), Eur. J. Biochem., 61, 259-269.
    View publication on PubMed
Search for more literature for 1.14.13.6

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration Pseudomonas putida
70000
-
 1 * 70000, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 ORC
-
Pseudomonas putida ORC
-
 ORC
-

Purification (Commentary)

Purification (Comment) Organism
ORC, using protamine sulfate treatment, column chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75 and a second DEAE-cellulose column chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.7
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chlorophenol + NADH + O2 33% of activity with orcinol Pseudomonas putida ?
-
 ?
3-cresol + NADH + O2 11-14% of the activity with orcinol Pseudomonas putida 3-methylcatechol + NAD+ + H2O
-
 ?
3-cresol + NADH + O2 11-14% of the activity with orcinol Pseudomonas putida ORC 3-methylcatechol + NAD+ + H2O
-
 ?
3-ethylphenol + NADH + O2 30% of activity with orcinol Pseudomonas putida ?
-
 ?
3-trifluoromethylphenol + NADH + O2 94% of activity with orcinol Pseudomonas putida ?
-
 ?
3-trifluoromethylphenol + NADH + O2 94% of activity with orcinol Pseudomonas putida ORC ?
-
 ?
additional information
-
 Pseudomonas putida ?
-
 ?
additional information
-
 Pseudomonas putida ORC ?
-
 ?
orcinol + NADH + O2
-
 Pseudomonas putida 2,3,5-trihydroxytoluene + NAD+ + H2O
-
 ?
orcinol + NADH + O2
-
 Pseudomonas putida ORC 2,3,5-trihydroxytoluene + NAD+ + H2O
-
 ?
resorcinol + NADH + O2 23% of activity with orcinol Pseudomonas putida hydroxyquinol + NAD+ + H2O
-
 ?
resorcinol + NADH + O2 23% of activity with orcinol Pseudomonas putida ORC hydroxyquinol + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Pseudomonas putida
FAD 1 mol of FAD per mol of protein Pseudomonas putida
NADH
-
 Pseudomonas putida
NADPH
-
 Pseudomonas putida