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Literature summary for 1.14.13.59 extracted from

  • Plattner, H.J.; Pfefferle, P.; Romaguera, A.; Waschutza, S.; Dieckman, H.
    Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222 (1989), Biol. Met., 2, 1-5.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DL-2,3-Diaminopropionic acid stimulates, but remains unchanged Escherichia coli
DL-2,6-diaminopimelic acid stimulates, but remains unchanged Escherichia coli
L-Orn stimulates, but remains unchanged Escherichia coli
N6-Acetyl-L-Lys stimulates, but remains unchanged Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate 0.01 mM, 62% inhibition, reversed by dithiothreitol Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
NADH
-
Escherichia coli
0.105
-
L-Lys
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Escherichia coli
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 50000, SDS-PAGE Escherichia coli
200000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
containing the structural gene for the enzyme on a multicopy plasmid
-
Escherichia coli EN222
-
containing the structural gene for the enzyme on a multicopy plasmid
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.189
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-Aminoethyl-L-Cys + NADPH + O2 i.e. L-aminoethylcysteine Escherichia coli ?
-
?
(S)-2-Aminoethyl-L-Cys + NADPH + O2 i.e. L-aminoethylcysteine Escherichia coli EN222 ?
-
?
1,5-Diaminopentane + NADPH + O2
-
Escherichia coli ?
-
?
DL-4-Selenalysine + NADPH + O2
-
Escherichia coli ?
-
?
DL-4-Selenalysine + NADPH + O2
-
Escherichia coli EN222 ?
-
?
DL-Homocysteine + NADPH + O2
-
Escherichia coli ?
-
?
DL-Homocysteine + NADPH + O2
-
Escherichia coli EN222 ?
-
?
DL/DL-Allo-delta-hydroxylysine + NADPH + O2
-
Escherichia coli ?
-
?
L-Lys + NADH + O2
-
Escherichia coli ?
-
?
L-Lys + NADH + O2
-
Escherichia coli EN222 ?
-
?
L-Lys + NADPH + O2 specific for NADPH Escherichia coli N6-Hydroxy-L-Lys + NADP+ + H2O
-
?
L-Lys + NADPH + O2 specific for NADPH Escherichia coli EN222 N6-Hydroxy-L-Lys + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD contains 0.79 mol FAD per mol of subunit Escherichia coli
NADPH required Escherichia coli