Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DL-2,3-Diaminopropionic acid | stimulates, but remains unchanged | Escherichia coli | |
DL-2,6-diaminopimelic acid | stimulates, but remains unchanged | Escherichia coli | |
L-Orn | stimulates, but remains unchanged | Escherichia coli | |
N6-Acetyl-L-Lys | stimulates, but remains unchanged | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | 0.01 mM, 62% inhibition, reversed by dithiothreitol | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
NADH | - |
Escherichia coli | |
0.105 | - |
L-Lys | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Escherichia coli |
200000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
containing the structural gene for the enzyme on a multicopy plasmid | - |
Escherichia coli EN222 | - |
containing the structural gene for the enzyme on a multicopy plasmid | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.189 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-Aminoethyl-L-Cys + NADPH + O2 | i.e. L-aminoethylcysteine | Escherichia coli | ? | - |
? | |
(S)-2-Aminoethyl-L-Cys + NADPH + O2 | i.e. L-aminoethylcysteine | Escherichia coli EN222 | ? | - |
? | |
1,5-Diaminopentane + NADPH + O2 | - |
Escherichia coli | ? | - |
? | |
DL-4-Selenalysine + NADPH + O2 | - |
Escherichia coli | ? | - |
? | |
DL-4-Selenalysine + NADPH + O2 | - |
Escherichia coli EN222 | ? | - |
? | |
DL-Homocysteine + NADPH + O2 | - |
Escherichia coli | ? | - |
? | |
DL-Homocysteine + NADPH + O2 | - |
Escherichia coli EN222 | ? | - |
? | |
DL/DL-Allo-delta-hydroxylysine + NADPH + O2 | - |
Escherichia coli | ? | - |
? | |
L-Lys + NADH + O2 | - |
Escherichia coli | ? | - |
? | |
L-Lys + NADH + O2 | - |
Escherichia coli EN222 | ? | - |
? | |
L-Lys + NADPH + O2 | specific for NADPH | Escherichia coli | N6-Hydroxy-L-Lys + NADP+ + H2O | - |
? | |
L-Lys + NADPH + O2 | specific for NADPH | Escherichia coli EN222 | N6-Hydroxy-L-Lys + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 0.79 mol FAD per mol of subunit | Escherichia coli | |
NADPH | required | Escherichia coli |