KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
progesterone | - |
Ilyonectria destructans | |
0.0043 | - |
NADPH | with progesterone and O2 as cosubstrates | Ilyonectria destructans | |
0.07 | - |
O2 | with progesterone and NADPH as cosubstrates | Ilyonectria destructans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Ilyonectria destructans |
116000 | - |
gel filtration, gel electrophoresis | Ilyonectria destructans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ilyonectria destructans | - |
ATTC 11011 | - |
Ilyonectria destructans ATTC 11011 | - |
ATTC 11011 | - |
Purification (Comment) | Organism |
---|---|
- |
Ilyonectria destructans |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-80°C, Tris buffer, stable for more than 6 months, enzyme in crude extract | Ilyonectria destructans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-Pregnadiene-17alpha,21-diol-3,11,20-trione + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
11beta-Hydroxyprogesterone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
11beta-Hydroxyprogesterone + NADPH + O2 | - |
Ilyonectria destructans ATTC 11011 | ? | - |
? | |
17alpha-Hydroxyprogesterone + NADPH + O2 | - |
Ilyonectria destructans | androstenedione + acetate + NADP+ + H2O | - |
? | |
17alpha-Hydroxyprogesterone + NADPH + O2 | - |
Ilyonectria destructans ATTC 11011 | androstenedione + acetate + NADP+ + H2O | - |
? | |
18-Hydroxydeoxycorticosterone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
21-Deoxycortisol + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
Aldosterone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
androstenedione + NADPH + O2 | - |
Ilyonectria destructans | 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O | - |
? | |
androstenedione + NADPH + O2 | - |
Ilyonectria destructans ATTC 11011 | 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O | - |
? | |
Corticosterone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
Cortisol + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
Cortisone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
deoxycorticosterone + NADPH + O2 | - |
Ilyonectria destructans | ? | - |
? | |
deoxycorticosterone + NADPH + O2 | - |
Ilyonectria destructans ATTC 11011 | ? | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Ilyonectria destructans | Testosterone acetate + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Ilyonectria destructans ATTC 11011 | Testosterone acetate + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, SDS-PAGE | Ilyonectria destructans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
progesterone monooxygenase reaction | Ilyonectria destructans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, progesterone monooxygenase activity | Ilyonectria destructans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme contains 1.6 mol of FAD per mol of enzyme | Ilyonectria destructans |