Literature summary for 1.14.13.50 extracted from

Wang, H.; Tiirola, M.A.; Puhakka, J.A.; Kulomaa, M.S.
Production and characterization of the recombinant Sphingomonas chlorophenolica pentachlorophenol 4-monooxygenase (2001), Biochem. Biophys. Res. Commun., 289, 161-166.

Search for more literature for 1.14.13.50

Cloned(Commentary)

Cloned (Comment)	Organism
pcpB gene, expression of wild-type and mutant in Escherichia coli as glutathione-S-transferase fusion protein	Flavobacterium sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	introduction of tobacco etch virus protease cleavage site into the enzyme as a glutathione-S-transferase fusion protein	Flavobacterium sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	Pentachlorophenol	recombinant enzyme	Flavobacterium sp.

Organism

Organism	UniProt	Comment	Textmining
Flavobacterium sp.	-	identical with Sphingomonas chlorophenolica strain ATCC 39723	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant from Escherichia coli as glutathione-S-transferase fusion proteins	Flavobacterium sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pentachlorophenol + NADPH + H+ + O2	-	Flavobacterium sp.	2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O	-	?

Synonyms

Synonyms	Comment	Organism
PCP4MO	-	Flavobacterium sp.

Cofactor

Cofactor	Comment	Organism	Structure
FAD	recombinant enzyme has no FAD, activity only in presence of added FAD	Flavobacterium sp.

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Release 2023.1 (January 2023)