Cloned (Comment) | Organism |
---|---|
pcpB gene, expression in Escherichia coli and Flavobacterium sp. ATCC 39723 of wild-type and isogenic mutant, DNA sequence analysis | Flavobacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | targeted mutagenesis via allelic exchange, construction of isogenic mutant without PCP 4-monooxygenase activity and 2 cointegration mutants with slow activity | Flavobacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + 2 NADPH + H+ + O2 | Flavobacterium sp. | solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one | tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O | - |
? | |
pentachlorophenol + 2 NADPH + H+ + O2 | Flavobacterium sp. | wild-type and mutant strains | tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
strain ATCC 39723 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | broad substrate and catalytic reaction range | Flavobacterium sp. | ? | - |
? | |
pentachlorophenol + 2 NADPH + H+ + O2 | solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one | Flavobacterium sp. | tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O | - |
? | |
pentachlorophenol + 2 NADPH + H+ + O2 | wild-type and mutant strains | Flavobacterium sp. | tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O | - |
? | |
pentachlorophenol + NADPH + H+ + O2 | - |
Flavobacterium sp. | 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Flavobacterium sp. | |
NADPH | requirement | Flavobacterium sp. |