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Literature summary for 1.14.13.40 extracted from

  • Langkau, B.; Vock, P.; Massey, V.; Fuchs, G.; Ghisla, S.
    2-Aminobenzoyl-CoA monooxygenase/reductase. Evidence for two distinct loci catalyzing substrate monooxygenation and hydrogenation (1995), Eur. J. Biochem., 230, 676-685.
    View publication on PubMed
Search for more literature for 1.14.13.40

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 NADH
-
 Pseudomonas sp.
0.47
-
 N-ethylmaleimide
-
 Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. KB740
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity of isolated and reconstituted enzyme in different fractions after purification Pseudomonas sp.

Storage Stability

Storage Stability Organism
-196°C stable Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-CoA + NAD(P)H + O2 i.e. anthraniloyl-CoA Pseudomonas sp. 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+
-
 ?
2-aminobenzoyl-CoA + NAD(P)H + O2 i.e. anthraniloyl-CoA Pseudomonas sp. KB740 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+
-
 ?
2-hydroxybenzoyl-CoA + NAD(P)H + O2 i.e. salicoyl-CoA, weak substrate, is probably monooxygenated but not hydrogenated Pseudomonas sp. ?
-
 ?
2-hydroxybenzoyl-CoA + NAD(P)H + O2 i.e. salicoyl-CoA, weak substrate, is probably monooxygenated but not hydrogenated Pseudomonas sp. KB740 ?
-
 ?
anthraniloyl-CoA + NADH + H+ + O2
-
 Pseudomonas sp. 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+
-
 r
anthraniloyl-CoA + NADH + H+ + O2
-
 Pseudomonas sp. KB740 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+
-
 r
N-ethylmaleimide + NAD(P)H + H+
-
 Pseudomonas sp. N-ethylsuccinimide + NAD(P)+
-
 ?
N-ethylmaleimide + NAD(P)H + H+
-
 Pseudomonas sp. KB740 N-ethylsuccinimide + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
dimer three dimeric forms: 1. alpha,alpha, 2. alpha',alpha', 3. alpha,alpha' Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 50% loss of activity in 2 days when stored highly concentrated, in dilute solutions it loses 50% of activity in 2 min, NADH, FAD and 2-aminobenzoyl CoA enhance stability Pseudomonas sp.
25
-
 50% loss of activity in 2 days when stored highly concentrated Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD the three dimeric forms alphaalpha, alpha'alpha' and alphaalpha' differ in their mode of binding FAD Pseudomonas sp.
NADH
-
 Pseudomonas sp.