Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminobenzoyl-CoA | substrate inhibition above 0.15 mM | Pseudomonas sp. | |
4-hydroxymercuribenzoate | at least 90% inhibition at 0.2 mM | Pseudomonas sp. | |
5,5'-dithiobis(2-nitrobenzoate) | at least 90% inhibition at 0.2 mM | Pseudomonas sp. | |
AgNO3 | at least 90% inhibition at 0.01 mM | Pseudomonas sp. | |
HgSO4 | at least 90% inhibition at 0.1 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
O2 | value below | Pseudomonas sp. | |
0.025 | - |
2-aminobenzoyl-CoA | value below | Pseudomonas sp. | |
0.042 | - |
NADH | - |
Pseudomonas sp. | |
0.25 | - |
N-ethylmaleimide | - |
Pseudomonas sp. | |
0.5 | - |
NADPH | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
SDS-PAGE | Pseudomonas sp. |
170000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-CoA + NAD(P)H + O2 | Pseudomonas sp. | pathway of aerobic breakdown of 2-aminobenzoate | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? | |
2-aminobenzoyl-CoA + NAD(P)H + O2 | Pseudomonas sp. KB740 | pathway of aerobic breakdown of 2-aminobenzoate | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. KB740 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-196°C stable for at least 6 months | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-CoA + NAD(P)H + O2 | - |
Pseudomonas sp. | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? | |
2-aminobenzoyl-CoA + NAD(P)H + O2 | pathway of aerobic breakdown of 2-aminobenzoate | Pseudomonas sp. | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? | |
2-aminobenzoyl-CoA + NAD(P)H + O2 | - |
Pseudomonas sp. KB740 | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? | |
2-aminobenzoyl-CoA + NAD(P)H + O2 | pathway of aerobic breakdown of 2-aminobenzoate | Pseudomonas sp. KB740 | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? | |
anthraniloyl-CoA + NADH + H+ + O2 | - |
Pseudomonas sp. | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+ | - |
r | |
anthraniloyl-CoA + NADH + H+ + O2 | - |
Pseudomonas sp. KB740 | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+ | - |
r | |
maleimide + NAD(P)H | - |
Pseudomonas sp. | succinimide + NAD(P)+ | - |
? | |
maleimide + NAD(P)H | - |
Pseudomonas sp. KB740 | succinimide + NAD(P)+ | - |
? | |
N-ethylmaleimide + NAD(P)H + H+ | - |
Pseudomonas sp. | N-ethylsuccinimide + NAD(P)+ | - |
? | |
N-ethylmaleimide + NAD(P)H + H+ | - |
Pseudomonas sp. KB740 | N-ethylsuccinimide + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 85000 SDS-PAGE, probably alpha2 dimer, may exist in three dimeric forms: 1. alpha,alpha, 2. alpha',alpha', 3. alpha,alpha', where alpha' may be a subunit with a different conformation | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
unstable above | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70.8 | - |
2-aminobenzoyl-CoA | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
2-aminobenzoyl-CoA + NADH | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Pseudomonas sp. | |
FAD | approximately 2 mol noncovalently bound FAD per mol of enzyme | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. | |
NADPH | enzyme is less active with NADPH than with NADH | Pseudomonas sp. |