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Literature summary for 1.14.13.39 extracted from

  • Gao, Y.T.; Roman, L.J.; Martasek, P.; Panda, S.P.; Ishimura, Y.; Masters, B.S.
    Oxygen metabolism by endothelial nitric-oxide synthase (2007), J. Biol. Chem., 282, 28557-28565.
    View publication on PubMed
Search for more literature for 1.14.13.39

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin activates O2 consumption of eNOS Rattus norvegicus
L-arginine L-arginine strongly stimulates oxygen consumption of eNOS and inhibits that of nNOS, nonhydrolyzable L-arginine analogues are not stimulatory Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of eNOS in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,6,7,8-tetrahydrobiopterin quenches the uncoupled reactions and results in much less reactive oxygen species formation, whereas the presence of redox-incompetent 7,8-dihydrobiopterin demonstrates little quenching effect Rattus norvegicus
L-arginine L-arginine strongly stimulates oxygen consumption of eNOS and inhibits that of nNOS Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required by eNOS, activates Rattus norvegicus
Fe2+ a cytochrome P450 enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus
-
 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
additional information Rattus norvegicus eNOS uncoupling is known to be controlled by substrate/cofactor availability, and the uncoupled reactions play important roles under various physiological/pathological conditions, such as atherosclerosis and septic shock ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant eNOS from Escherichia coli by 2',5'-ADP affinity chromatography Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 first half reaction via intermediate Nomega-hydroxy-L-arginine Rattus norvegicus 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 complete reaction, oxygen stoichiometry, effects of substrate/cofactor binding on the endothelial NOS isoform, eNOS, overview Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
additional information eNOS uncoupling is known to be controlled by substrate/cofactor availability, and the uncoupled reactions play important roles under various physiological/pathological conditions, such as atherosclerosis and septic shock Rattus norvegicus ?
-
 ?
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 second half reaction via intermediate Nomega-hydroxy-L-arginine Rattus norvegicus citrulline + nitric oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
endothelial nitric-oxide synthase
-
 Rattus norvegicus
eNOS
-
 Rattus norvegicus
neuronal nitric-oxide synthase
-
 Rattus norvegicus
nNOS
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
24
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus