Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+/calmodulin | activate the electron transfer | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | biochemical pathway model, kinetic modeling, isozymes NOS1 and NOS3 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+/calmodulin | activate the electron transfer | Homo sapiens | |
O2 | oxygen tension influences the activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Homo sapiens | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozymes NOS1 and NOS3 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | reaction mechanism, NO release and transport, overview, NO formation through sequential electron transfer and the oxidation of L-arginine, in the catalytic process, FMN, FAD, and tetrahydrobiopterin are required as coenzymes, and the presence of Ca2+/calmodulin can aid the electron transfer, isozymes NOS1 and NOS3 perform a similar reaction mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
arteriole | isozyme NOS3 | Homo sapiens | - |
mast cell | isozyme NOS1 | Homo sapiens | - |
nerve | isozyme NOS1 | Homo sapiens | - |
neuron | isozyme NOS1 | Homo sapiens | - |
smooth muscle | isozyme NOS3 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endothelial nitric oxide synthase | - |
Homo sapiens |
neuronal nitric oxide synthase | - |
Homo sapiens |
NOS1 | - |
Homo sapiens |
NOS3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required | Homo sapiens | |
FMN | required | Homo sapiens | |
NADPH | required | Homo sapiens | |
tetrahydrobiopterin | required | Homo sapiens |