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Literature summary for 1.14.13.39 extracted from

  • Chandok, M.R.; Ytterberg, A.J.; van Wijk, K.J.; Klessig, D.F.
    The pathogen-inducible nitric oxide synthase (iNOS) in plants is a variant of the P protein of the glycine decarboxylase complex (2003), Cell, 113, 469-482.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin required Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
aminoguanidine
-
Nicotiana tabacum
Ngamma,Ngamma-dimethyl-L-arginine
-
Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000383
-
NADPH pH 7.0, 37°C Nicotiana tabacum
0.00746
-
L-arginine pH 7.0, 37°C Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
x * 120000 + x * 115000, SDS-PAGE Nicotiana tabacum
120000
-
x * 120000 + x * 115000, SDS-PAGE Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf leaf extract Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
983.7
-
pH 7.0, 37°C Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Nicotiana tabacum 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?

Subunits

Subunits Comment Organism
? x * 120000 + x * 115000, SDS-PAGE Nicotiana tabacum

Synonyms

Synonyms Comment Organism
iNOS
-
Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
Nicotiana tabacum
FAD
-
Nicotiana tabacum
NADPH
-
Nicotiana tabacum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00667
-
Ngamma,Ngamma-dimethyl-L-arginine pH 7.0, 37°C Nicotiana tabacum