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Literature summary for 1.14.13.39 extracted from
- Characterization of Drosophila nitric oxide synthase: a biochemical study (2003), Biochem. Biophys. Res. Commun., 306, 590-597.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | substrate 2,6-dichlorophenolindophenol, about 10fold increase in activity in presence of calmodulin | Drosophila melanogaster |  |
General Stability
| General Stability | Organism |
|---|---|
| very stable against trypsin, reductase domain is much more susceptible to urea than oxygenase domain | Drosophila melanogaster |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 152000 | - |
x * 152000, SDS-PAGE, plus a proteolytic artefact of 121000 | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
expression in Escherichia coli | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| flavoprotein | - |
Drosophila melanogaster |
| additional information | hemoprotein | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-dichlorophenolindophenol + NADPH + O2 | best substrate, about 10-fold increase in activity in presence of calmodulin | Drosophila melanogaster | ? + NO + NADP+ | - |
? | |
| ferricyanide + NADPH + O2 | - |
Drosophila melanogaster | ferrocyanide + NADP+ + ? | - |
? | |
| ferricytochrome c + NADPH + O2 | - |
Drosophila melanogaster | ferrocytochrome c + NO + NADP+ | - |
? | |
| L-arginine + H2O2 + tetrahydrobiopterin | - |
Drosophila melanogaster | ? + NO + NADP+ | - |
? | |
| L-arginine + NADPH + O2 + tetrahydrobiopterin | - |
Drosophila melanogaster | citrulline + NO + NADP+ + ? | - |
? | |
| N-hydroxy-L-arginine + H2O2 + tetrahydrobiopterin | - |
Drosophila melanogaster | ? + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 152000, SDS-PAGE, plus a proteolytic artefact of 121000 | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNOS | - |
Drosophila melanogaster |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,6,7,8-tetrahydro-L-biopterin | - |
Drosophila melanogaster | |
| NADPH | - |
Drosophila melanogaster | |
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