Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of neuronal isoform wild type and mutants C415H, C415A in Spodoptera frugiperda cells via baculovirus infection | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C415A | contains no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c | Mus musculus |
C415H | contains nearly no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Mus musculus | |
Iron | heme ligand is bound via C415 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutants from insect cells | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type and mutants | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
oxidized cytochrome c + NADPH + O2 | wild-type and mutants | Mus musculus | reduced cytochrome c + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | only wild-type | Mus musculus | |
Calmodulin | Ca2+/calmodulin stimulates cytochrome c reductase activity | Mus musculus | |
Calmodulin | 15fold stimulation of cytochrome c reduction of wild-type and mutants C415A and C415H | Mus musculus | |
FAD | wild-type and mutant C415H contain1 mol per mol of subunit | Mus musculus | |
FMN | wild-type and mutant C415H contain 0.8 and 0.9 mol per mol of subunit, respectively | Mus musculus | |
NADPH | - |
Mus musculus |