Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | protoporphyrin IX heme | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, oxygenase subunit domain, SDS-PAGE | Mus musculus |
74000 | - |
reductase subunit domain, gel filtration | Mus musculus |
112000 | - |
oxygenase subunit domain, gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
dimeric enzyme and subunits | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | subunits align in head-to-head manner with oxygenase domains interacting to form a dimer and reductase domains existing as independent extensions | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | RAW 264.7 cells | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
purified enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
oxidized cytochrome c + NADPH + O2 | - |
Mus musculus | reduced cytochrome c + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, oxygenase subunit domain, SDS-PAGE | Mus musculus |
monomer | 1 * 74000, reductase domain, SDS-PAGE | Mus musculus |
More | dimer formation, each subunit consists of: 1 oxygenase domain containing heme, tetrahydrobiopterin, substrate binding site and 1 reductase domain containing FAD, FMN, calmodulin, NADPH binding site | Mus musculus |
More | dimeric structure is required for enzyme activity, interaction between subunits via oxygenase domains | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | required | Mus musculus | |
FAD | - |
Mus musculus | |
FMN | - |
Mus musculus | |
NADPH | - |
Mus musculus |