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Literature summary for 1.14.13.39 extracted from
- Identification and characterization of a calmodulin-dependent nitric oxide synthase from GH3 pituitary cells (1992), Biochem. J., 285, 201-206.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ngamma-monomethyl-L-arginine | inhibits citrulline formation, not cytochrome c reduction | Bos taurus |  |
| Ngamma-nitro-L-arginine | inhibits citrulline formation, not cytochrome c reduction | Bos taurus | |
| nitroblue tetrazolium | - |
Bos taurus | |
| Trifluoperazine | inhibits cytochrome c reductase activity | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000004 | - |
Calmodulin | - |
Bos taurus | |
| 0.0004 | - |
NADPH | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation only in the presence of calmodulin | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 152000 | - |
x * 152000, SDS-PAGE, Western Blot | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pituitary gland | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 152000, SDS-PAGE, Western Blot | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 5,6,7,8-tetrahydro-L-biopterin | stimulates 9fold | Bos taurus | |
| Calmodulin | Ca2+/calmodulin stimulates cytochrome c reductase activity | Bos taurus | |
| NADPH | - |
Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
Ngamma-nitro-L-arginine | - |
Bos taurus | |
| 0.0018 | - |
Ngamma-monomethyl-L-arginine | - |
Bos taurus | |
| 0.007 | - |
nitroblue tetrazolium | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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