Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ngamma-iminoethyl-L-ornithine | competitive inhibitor | Rattus norvegicus | |
Ngamma-monomethyl-L-arginine | not D-isomer, strong, competitive | Rattus norvegicus | |
Ngamma-nitro-L-arginine | competitive inhibitor | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | 0.0084 | dichlorophenolindophenol | with L-arginine | Rattus norvegicus | |
0.0084 | - |
L-arginine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | NO represents the endogenous activator of soluble guanylyl cyclase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
forebrain | synaptosomal fraction | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000043 | - |
crude extract | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound | ? | |
2,6-dichlorophenolindophenol + NADPH + O2 | - |
Rattus norvegicus | ? + NO + NADP+ | - |
? | |
additional information | NO represents the endogenous activator of soluble guanylyl cyclase | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Rattus norvegicus | |
NADPH | crude preparation requires only NADPH as cofactor | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
Ngamma-nitro-L-arginine | - |
Rattus norvegicus | |
0.0007 | - |
Ngamma-monomethyl-L-arginine | - |
Rattus norvegicus | |
0.0012 | - |
Ngamma-iminoethyl-L-ornithine | - |
Rattus norvegicus |