General Stability | Organism |
---|---|
freezing inactivates, 50% v/v glycerol protects | Mus musculus |
glycerol stabilizes | Mus musculus |
Mg2+ does not stabilize | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | 0.018 | L-arginine | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
2 * 130000, SDS-PAGE | Mus musculus |
260000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
cytokine-inducible in macrophage | - |
Purification (Comment) | Organism |
---|---|
inducible form from macrophage | Mus musculus |
sequential anion-exchange-, affinity chromatogaphy | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.62 | - |
purified enzyme | Mus musculus |
Storage Stability | Organism |
---|---|
-80°C, stable overnight in 50% v/v glycerol | Mus musculus |
4°C, t1/2: 2 h | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 130000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | presumably tightly enzyme-bound | Mus musculus | |
FAD | 1 mol per mol of enzyme subunit | Mus musculus | |
FAD | FAD containing flavoprotein | Mus musculus | |
FAD | slight activation by exogeneous FAD | Mus musculus | |
FMN | 1 mol per mol of enzyme subunit | Mus musculus | |
FMN | FMN containing flavoprotein | Mus musculus | |
NADPH | - |
Mus musculus |