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Literature summary for 1.14.13.39 extracted from

  • Klatt, P.; Heinzel, B.; John, M.; Kastner, M.; Böhme, E.; Mayer, B.
    Ca2+/calmodulin-dependent cytochrome c reductase activity of brain nitric oxide synthase (1992), J. Biol. Chem., 267, 11374-11378.
    View publication on PubMed
Search for more literature for 1.14.13.39

Inhibitors

Inhibitors Comment Organism Structure
nitroblue tetrazolium
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008 0.0084 dichlorophenolindophenol
-
 Sus scrofa
0.016
-
 nitroblue tetrazolium
-
 Sus scrofa
0.0323 0.035 L-arginine cytochrome c reduction Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme bears Ca2+/calmodulin dependent cytochrome P-450 reductase activity which catalyzes cytochrome c reduction Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sus scrofa NO represents the endogenous activator of soluble guanylyl cyclase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Sus scrofa
-
cerebellum
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 reductase activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Sus scrofa 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound ?
2,6-dichlorophenolindophenol + NADPH + O2
-
 Sus scrofa ? + NO + NADP+
-
 ?
additional information the reductase domain has a broad substrate specificity, catalyzes a moderate Ca2+/calmodulin independent hydroxylation when the enzyme is reconstituted with purified P-450 Sus scrofa ?
-
 ?
additional information NO represents the endogenous activator of soluble guanylyl cyclase Sus scrofa ?
-
 ?
nitroblue tetrazolium + 2 NADPH
-
 Sus scrofa nitroblue tetrazolium formazan + 2 NADP+
-
 ?
oxidized cytochrome c + NADPH + O2 reaction is enhanced by addition of calmodulin at 0.0002 mM Sus scrofa reduced cytochrome c + NADP+ + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
2,6-dichlorophenolindophenol activation Sus scrofa
5,6,7,8-tetrahydro-L-biopterin
-
 Sus scrofa
Calmodulin the enzyme bears a Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors Sus scrofa
cytochrome c activation Sus scrofa
FAD the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors Sus scrofa
FMN the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors Sus scrofa
NADPH
-
 Sus scrofa
nitroblue tetrazolium activation Sus scrofa