Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.35 extracted from

  • Rao, P.V.S.; Sreeleela, N.S.; Kumar, R.P.; Vaidyanathan, C.S.
    Anthranilic acid hydroxylase (Aspergillus niger) (1970), Methods Enzymol., 17A, 510-513.
No PubMed abstract available
Search for more literature for 1.14.13.35

General Stability

General Stability Organism
glutathione stabilizes Aspergillus niger
the enzyme is inactivated by treatment with ammonium sulfate or by adsorption on DEAE-cellulose or CM-cellulose, filtration through Sephadex G-25 or dialysis against 0.025 M sodium phosphate buffer, pH 7, containing 1 mM GSH irreversibly inactivates the enzyme Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Aspergillus niger
2,2'-dipyridyl
-
 Aspergillus niger
diethyldithiocarbamate
-
 Aspergillus niger
heavy metal ions
-
 Aspergillus niger
N-ethylmaleimide
-
 Aspergillus niger
NaN3
-
 Aspergillus niger
p-chloromercuribenzoate
-
 Aspergillus niger
Salicylaldoxime
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 anthranilic acid
-
 Aspergillus niger
0.16
-
 NADPH
-
 Aspergillus niger

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone inactivates the enzyme Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using protamine sulfate treatment, DEAE-cellulose treatment and alumina C-gamma treatment Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus niger

Storage Stability

Storage Stability Organism
-20°C, 50% inactivation of the purified enzyme after 2 days Aspergillus niger
frozen, mycelium, no appreciable loss of activity after 1 week Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyanthranilate + NADPH + O2 no activity Aspergillus niger ?
-
 ?
3-methylanthranilate + NADPH + O2 no activity Aspergillus niger ? + NADP+ + NH3
-
 ?
anthranilate + NADPH + O2
-
 Aspergillus niger 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?
additional information no activity with: 3-hydroxyanthranilic acid, benzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, m-aminobenzoic acid, p-aminobenzoic acid, methylanthranilate or ethylanthranilate Aspergillus niger ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 at pH 7.5 or pH 9.0 about 60% of activity maximum Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
additional information 2% of the activity when NADPH is replaced by NADH, no effect: FAD, FMN Aspergillus niger
NADPH absolute requirement for NADPH Aspergillus niger