Cloned (Comment) | Organism |
---|---|
genes onpA and onpB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning and expression in Escherichia coli, co-expression with onpB, but not of onpA alone, in the catechol utilizer Pseudomonas putida strain PaW340 confers the ability to grow on 2-nitrophenol | Alcaligenes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | highly activating | Alcaligenes sp. | |
additional information | the enzyme activity is higher in phosphate buffer than in Tris-HCl buffer at pH 7.5 | Alcaligenes sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenol + NADPH + H+ + O2 | Alcaligenes sp. | - |
catechol + NADP+ + H2O + nitrite | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | Alcaligenes sp. NyZ215 | - |
catechol + NADP+ + H2O + nitrite | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | A7KS54 | ene onpA encoded in the onpABC operon | - |
Alcaligenes sp. NyZ215 | A7KS54 | ene onpA encoded in the onpABC operon | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the organism is able to grow on ortho-nitrophenol as the sole source of carbon, nitrogen, and energy | Alcaligenes sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-benzoquinone + NADPH + H+ | reaction step 2 catalzed by OnpB | Alcaligenes sp. | catechol + NADP+ | - |
? | |
1,2-benzoquinone + NADPH + H+ | reaction step 2 catalzed by OnpB | Alcaligenes sp. NyZ215 | catechol + NADP+ | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | - |
Alcaligenes sp. | catechol + NADP+ + H2O + nitrite | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | overall reaction by OnpAB, both OnpA and OnpB are required for catechol formation, overview | Alcaligenes sp. | catechol + NADP+ + H2O + nitrite | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | - |
Alcaligenes sp. NyZ215 | catechol + NADP+ + H2O + nitrite | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | overall reaction by OnpAB, both OnpA and OnpB are required for catechol formation, overview | Alcaligenes sp. NyZ215 | catechol + NADP+ + H2O + nitrite | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | reaction step 1 catalzed by OnpA | Alcaligenes sp. | 2-benzoquinone + NADP+ + H2O + nitrite | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | reaction step 1 catalzed by OnpA | Alcaligenes sp. NyZ215 | 2-benzoquinone + NADP+ + H2O + nitrite | - |
? | |
4-methyl-2-nitrophenol + NADPH + H+ + O2 | 108% of the activity with 2-nitrophenol | Alcaligenes sp. | 4-methyl-catechol + NADP+ + H2O + nitrite | - |
? | |
4-methyl-2-nitrophenol + NADPH + H+ + O2 | 108% of the activity with 2-nitrophenol | Alcaligenes sp. NyZ215 | 4-methyl-catechol + NADP+ + H2O + nitrite | - |
? | |
5-fluoro-2-nitrophenol + NADPH + H+ + O2 | about 20% of the activity with 2-nitrophenol | Alcaligenes sp. | 5-fluoro-catechol + NADP+ + H2O + nitrite | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ONP 2-monooxygenase | - |
Alcaligenes sp. |
OnpA | - |
Alcaligenes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Alcaligenes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Alcaligenes sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | slightly activating, bound to the enzyme | Alcaligenes sp. | |
NADPH | - |
Alcaligenes sp. |