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Literature summary for 1.14.13.31 extracted from

  • Kadiyala, V.; Spain, J.C.
    A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and the oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905 (1998), Appl. Environ. Microbiol., 64, 2479-2484.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-nitrophenol + NADPH + O2 Lysinibacillus sphaericus
-
?
-
?
2-nitrophenol + NADPH + O2 Lysinibacillus sphaericus JS905
-
?
-
?

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-
Lysinibacillus sphaericus JS905
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
-
Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenol + NADPH + H+ + O2
-
Lysinibacillus sphaericus 3-nitrocatechol + NADP+ + H2O
-
?
2-nitrophenol + NADPH + H+ + O2
-
Lysinibacillus sphaericus JS905 3-nitrocatechol + NADP+ + H2O
-
?
2-nitrophenol + NADPH + O2
-
Lysinibacillus sphaericus ?
-
?
2-nitrophenol + NADPH + O2
-
Lysinibacillus sphaericus JS905 ?
-
?
4-nitroresorcinol + NADPH + O2
-
Lysinibacillus sphaericus ?
-
?
4-nitroresorcinol + NADPH + O2
-
Lysinibacillus sphaericus JS905 ?
-
?
additional information 2-component enzyme system comprising of a flavoprotein reductase and an oxygenase Lysinibacillus sphaericus ?
-
?
additional information 2-component enzyme system comprising of a flavoprotein reductase and an oxygenase Lysinibacillus sphaericus JS905 ?
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Lysinibacillus sphaericus
NADH
-
Lysinibacillus sphaericus
NADPH
-
Lysinibacillus sphaericus