KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0116 | - |
NADH | pH 7.2, 36°C | Methylomonas methanica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | as a true halotolerant enzyme, MmoC still shows 50% of its specific activity at 2 M NaCl. Evaluation of salt tolerance for NADH-mediated reduction of benzyl viologen by MmoC at non-optima conditions, 23°C and pH 7.0 | Methylomonas methanica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NADH + H+ + O2 | Methylomonas methanica | - |
methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | Methylomonas methanica MC09 | - |
methanol + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylomonas methanica | - |
- |
- |
Methylomonas methanica MC09 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NADH + H+ + O2 | - |
Methylomonas methanica | methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | - |
Methylomonas methanica MC09 | methanol + NAD+ + H2O | - |
? | |
additional information | the soluble methane monooxygenase receives electrons from NADH via its reductase MmoC for oxidation of methane. The NADH-dependent reductase MmoC produces only trace amounts of superoxide, but mainly hydrogen peroxide during uncoupled turnover reactions | Methylomonas methanica | ? | - |
- |
|
additional information | the soluble methane monooxygenase receives electrons from NADH via its reductase MmoC for oxidation of methane. The NADH-dependent reductase MmoC produces only trace amounts of superoxide, but mainly hydrogen peroxide during uncoupled turnover reactions | Methylomonas methanica MC09 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 37900, SDS-PAGE | Methylomonas methanica |
Synonyms | Comment | Organism |
---|---|---|
MmMmoC | - |
Methylomonas methanica |
MmoC | - |
Methylomonas methanica |
sMMO | - |
Methylomonas methanica |
soluble methane monooxygenase | - |
Methylomonas methanica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
- |
Methylomonas methanica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the MmoC-related turnover rate or the NADH:BV oxidoreductase activity is 4585/min with an apparent molecular weight of 37.9 kDa. The hydrogen peroxide production activity of MmMmoC is 0.14 U/mg, the turnover rate of 5.2/min | Methylomonas methanica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Methylomonas methanica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the specific activity of MmoC is increased significantly by 20% in presence of 25 microM FAD, whereas addition of FMN does not improve activity confirming FAD as the prosthetic group | Methylomonas methanica | |
additional information | presence of a FAD and a [2Fe2S] cluster as its prosthetic group at the reductase MmoC component of the soluble methane monooxygenase complex. The MmMmoC prefers NADH instead of NADPH. No NADPH oxidation activity can be determined at 1 mM | Methylomonas methanica | |
NADH | specific for | Methylomonas methanica |
General Information | Comment | Organism |
---|---|---|
additional information | MmoC homology modeling using structure PDB ID 1KRH.1 and the crystal structure of the monomeric MMOH-MmoB complex from Methylococcus capsulatus (PDB ID 4GAM) as a templates | Methylomonas methanica |