Application | Comment | Organism |
---|---|---|
environmental protection | pulping wastewater still contains massive refractory organics after biotreatment, with high colority, low biodegradability, and lasting biotoxicity. To eliminate refractory organics in pulping wastewater, a methanotrophic co-metabolic system in a gas cycle Sequencing Batch Biofilm Reactor (gcSBBR) seeded by soil at a ventilation opening of coal mine is quickly built on the 92nd day. The removal rate of COD, colority and TOC is 53.28%, 50.59% and 51.60%, respectively. Analysis of 3D-EEM indicates that glycolated protein-like, melanoidin-like or lignocellulose-like, and humic acid-like decrease by 7.85%, 5.02% and 1.74%, respectively | Methylosinus trichosporium |
Cloned (Comment) | Organism |
---|---|
gene mmoX, quantitative RT-PCR enzyme expression analysis | Methylosinus trichosporium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | establishing of a methanotrophic co-metabolic system, built in the gcSBBR seeded by soil at a ventilation opening of coal mine in the presence of Cu2+, and seeded with sMMO in Methylosinus trichosporium OB3b and particulate methane monooxygenase (pMMO) in Methylocystis parvus in a biofilm, microbial community analyses and microbial 16S rRNA sequencing, and postulated pathway of methanotrophic co-metabolic system, overview. Six kinds of methanotrophs are detected from the seeded soil, namely, Methylocystis (0.29%), Methylocystaceae_unclassified (0.16%), Methylocystaceae_uncultured (0.04%) and Methylocaldum (0.14%), Methylococcaceae_unclassified (0.10%), Methylobacteriaceae_uncultured (0.04%), totally accounting for 0.77% | Methylosinus trichosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NAD(P)H + H+ + O2 | Methylosinus trichosporium | - |
methanol + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylosinus trichosporium | A0A2D2D5X0 AND A0A2D2D0T8 AND Q53563 AND A0A2D2D0X7 | MMOH, MMOR, MMOB, and MMOD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
additional information | a colorimetric assay is adopted for the sMMO activity detection of biofilm | Methylosinus trichosporium | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
sMMO | - |
Methylosinus trichosporium |
soluble methane monooxygenase | - |
Methylosinus trichosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Methylosinus trichosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Methylosinus trichosporium | |
NAD(P)H | - |
Methylosinus trichosporium |