KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | analysis of kinetics of single turnover reactions of the two intermediates with ethyl vinyl ether and diethyl ether, single- and double-mixing stopped-flow optical spectroscopy, overview, stopped-flow kinetics, analytical model of transient kinetics | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Methylococcus capsulatus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NADH + O2 | Methylococcus capsulatus | - |
methanol + NAD+ + H2O | - |
? | |
methane + NADH + O2 | Methylococcus capsulatus Bath | - |
methanol + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Methylococcus capsulatus Bath | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native MMOH, in the purification procedure includes anion exchange chromatography and gel filtration | Methylococcus capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | the soluble enzyme utilizes a carboxylate-bridged diiron center and dioxygen, a di(my-oxo)diiron(IV) intermediate termed Q is responsible for the catalytic activity with hydrocarbons, the peroxodiiron(III) intermediate precedes Q formation in the catalytic cycle | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | 0.04 | purified MMOH, substrate propylene | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NADH + O2 | - |
Methylococcus capsulatus | methanol + NAD+ + H2O | - |
? | |
methane + NADH + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD+ + H2O | - |
? | |
propylene + NADH + O2 | the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene | Methylococcus capsulatus | propylene oxide + NAD+ + H2O | - |
? | |
propylene + NADH + O2 | the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene | Methylococcus capsulatus Bath | propylene oxide + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MMOH | - |
Methylococcus capsulatus |
soluble methane monooxygenase hydroxylase | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |