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Literature summary for 1.14.13.25 extracted from

  • Beauvais, L.G.; Lippard, S.J.
    Reactions of the peroxo intermediate of soluble methane monooxygenase hydroxylase with ethers (2005), J. Am. Chem. Soc., 127, 7370-7378.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information analysis of kinetics of single turnover reactions of the two intermediates with ethyl vinyl ether and diethyl ether, single- and double-mixing stopped-flow optical spectroscopy, overview, stopped-flow kinetics, analytical model of transient kinetics Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Methylococcus capsulatus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NADH + O2 Methylococcus capsulatus
-
methanol + NAD+ + H2O
-
?
methane + NADH + O2 Methylococcus capsulatus Bath
-
methanol + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus Bath
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native MMOH, in the purification procedure includes anion exchange chromatography and gel filtration Methylococcus capsulatus

Reaction

Reaction Comment Organism Reaction ID
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O the soluble enzyme utilizes a carboxylate-bridged diiron center and dioxygen, a di(my-oxo)diiron(IV) intermediate termed Q is responsible for the catalytic activity with hydrocarbons, the peroxodiiron(III) intermediate precedes Q formation in the catalytic cycle Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02 0.04 purified MMOH, substrate propylene Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NADH + O2
-
Methylococcus capsulatus methanol + NAD+ + H2O
-
?
methane + NADH + O2
-
Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
?
propylene + NADH + O2 the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene Methylococcus capsulatus propylene oxide + NAD+ + H2O
-
?
propylene + NADH + O2 the peroxodiiron(III) intermediate that precedes Q formation in the catalytic cycle has been demonstrated to react with propylene Methylococcus capsulatus Bath propylene oxide + NAD+ + H2O
-
?

Synonyms

Synonyms Comment Organism
MMOH
-
Methylococcus capsulatus
soluble methane monooxygenase hydroxylase
-
Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
assay at Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylococcus capsulatus