Cloned (Comment) | Organism |
---|---|
DNA sequence analysis | Methylococcus capsulatus |
overexpression of an additional protein component D of sMMO encoded by orfY as thioredoxin-fusion protein with His-Tag in Escherichia coli, protein component D is termed MMOD | Methylococcus capsulatus |
General Stability | Organism |
---|---|
sMMO is more stable and easier to purify than pMMO | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | sMMO is completely inhibited by monomeric component D, but not by dimeric, monomeric MMOD is interfering with the catalytically active complex between component A hydroxylase and component protein B | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cytoplasmatic, soluble enzyme form termed sMMO | Methylococcus capsulatus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
component D of sMMO: 2 * 12000, SDS-PAGE | Methylococcus capsulatus |
24000 | - |
component D of sMMO, forms homodimers, gel filtration | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
Bath | - |
Methylococcus capsulatus Bath | - |
Bath | - |
Purification (Comment) | Organism |
---|---|
component D of sMMO recombinant from Escherichia coli as His-tagged thioredoxin-fusion protein, the thioredoxin is cleaved off during purification by factor Xa | Methylococcus capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | modeling of interaction between the different protein components of sMMO | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? | |
propylene + NAD(P)H + O2 | enzyme form sMMO | Methylococcus capsulatus | propylene oxide + NADP+ + H2O | - |
? | |
propylene + NAD(P)H + O2 | enzyme form sMMO | Methylococcus capsulatus Bath | propylene oxide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Methylococcus capsulatus |
dimer | component D of sMMO: 2 * 12000, SDS-PAGE | Methylococcus capsulatus |
More | sMMO consists of 4 components: a hydroxylase, a reductase, a protein B and a protein D | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
MMO | - |
Methylococcus capsulatus |
pMMO | particulate, membrane-bound enzyme form | Methylococcus capsulatus |
sMMO | soluble, cytoplasmic enzyme form | Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methylococcus capsulatus |
25 | - |
enzyme form sMMO | Methylococcus capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methylococcus capsulatus |
7 | - |
enzyme form sMMO | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | protein C, reductase component: contains 1 mol FAD per mol protein | Methylococcus capsulatus | |
additional information | component D contains no metal ions or organic cofactors | Methylococcus capsulatus | |
NADH | - |
Methylococcus capsulatus |