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Literature summary for 1.14.13.25 extracted from

  • Pilkington, S.J.; Dalton, H.
    Purification and characterization of the soluble methane monooxygenase from Methylosinus sporium 5 demonstrates the highly conserved nature of this enzyme in methanotrophs (1991), FEMS Microbiol. Lett., 78, 103-108.
No PubMed abstract available
Search for more literature for 1.14.13.25

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Methylosinus sporium 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 2.1 mol Fe per mol enzyme Methylosinus sporium
Iron 1 mol Fe per mol enzyme Methylosinus sporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 see under subunits: molecular weights of the subunits of components Methylosinus sporium
additional information
-
 enzyme system consists of 3 protein components A, B, C Methylosinus sporium
20000
-
 component A: 2 * 56000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE Methylosinus sporium
40000
-
 component C acceptor reductase Methylosinus sporium
56000
-
 component A: 2 * 56000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE Methylosinus sporium
220000
-
 component A: hydroxylase, gel filtration Methylosinus sporium

Organism

Organism UniProt Comment Textmining
Methylosinus sporium
-
-
-
Methylosinus sporium 5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protein A and C Methylosinus sporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.185
-
 component A Methylosinus sporium
17.5
-
 component C Methylosinus sporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NAD(P)H + O2
-
 Methylosinus sporium methanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2
-
 Methylosinus sporium 5 methanol + NAD(P)+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? component A: 2 * 56000 alpha + 2 * 40000 beta + 2 * 20000 gamma, SDS-PAGE Methylosinus sporium
More see under molecular weight for the size of the protein components Methylosinus sporium
More enzyme system consists of 3 protein components A, B, C Methylosinus sporium

Synonyms

Synonyms Comment Organism
MMO
-
 Methylosinus sporium

Cofactor

Cofactor Comment Organism Structure
FAD protein C, reductase component: contains 1 mol FAD per mol protein Methylosinus sporium
NADH
-
 Methylosinus sporium