Cloned (Comment) | Organism |
---|---|
expression in Rhodococcus jostii RHA#2 | Rhodococcus jostii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | Q0SFK6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus jostii |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxybenzoate 6-hydroxylase | - |
Rhodococcus jostii |
3HB6H | - |
Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Rhodococcus jostii | |
additional information | the enzyme harbors a phospholipid ligand. The purified enzyme obtained from expressing the gene encoding 3HB6H from Rhodococcus jostii RHA1 in the host Escherichia coli contains a mixture of phosphatidylglycerol and phosphatidylethanolamine, which are the major constituents of the cytoplasmic membrane of Escherichia coli. The enzyme that is produced in the host Rhodococcus jostii RHA#2 by employing a newly developed actinomycete expression system, contains phosphatidylinositol, which is a specific constituent of actinomycete membranes. The lipid cofactor stabilizes monomer-monomer contact | Rhodococcus jostii |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes a key step in the catabolism of lignin-derived aromatic compounds | Rhodococcus jostii |