Literature summary for 1.14.13.24 extracted from

Sucharitakul, J.; Medhanavyn, D.; Pakotiprapha, D.; van Berkel, W.J.; Chaiyen, P.
Tyr217 and His213 are important for substrate binding and hydroxylation of 3-hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1 (2016), FEBS J., 283, 860-881 .

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Protein Variants

Protein Variants	Comment	Organism
H213A	the mutant enzyme cannot catalyze hydroxylation	Rhodococcus jostii
H213D	the mutant enzyme can bind to 3-hydroxybenzoate with similar affinity as the wild-type enzyme and form C4a-hydroperoxy intermediate. It produces 2,5-dihydroxybenzoate with yields of 52%	Rhodococcus jostii
H213E	the mutant enzyme can bind to 3-hydroxybenzoate with similar affinity as the wild-type enzyme and form C4a-hydroperoxy intermediate. It produces 2,5-dihydroxybenzoate with yields of 92%. The hydroxylation rate constant of the mutant enzyme (35/s) is similar to that of wild-type enzyme (36/s) and this variant has an efficiency of hydroxylation (92%) similar to the wild-type enzyme (86%)	Rhodococcus jostii
H213S	the mutant enzyme can bind to 3-hydroxybenzoate with similar affinity as the wild-type enzyme and form C4a-hydroperoxy intermediate. It produces 2,5-dihydroxybenzoate with yields of 28%	Rhodococcus jostii
Y217A	the mutant enzyme does not show any perturbation of flavin absorption upon addition of 3-hydroxybenzoate	Rhodococcus jostii
Y217F	the mutant enzyme has a Kd value for 3-hydroxybenzoate binding of 7.5 mM, which is about 50fold larger than that found for wild-type enzyme. The results indicate that Tyr217 is necessary for substrate binding	Rhodococcus jostii
Y217S	the mutant enzyme does not show any perturbation of flavin absorption upon addition of 3-hydroxybenzoate	Rhodococcus jostii

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus jostii	Q0SFK6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxybenzoate + NADH + H+ + O2	-	Rhodococcus jostii	2,5-dihydroxybenzoate + NAD+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
3-hydroxybenzoate 6-hydroxylase	-	Rhodococcus jostii
3HB6H	-	Rhodococcus jostii

Cofactor

Cofactor	Comment	Organism	Structure
FAD	flavoprotein	Rhodococcus jostii

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Release 2023.1 (January 2023)