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Literature summary for 1.14.13.24 extracted from
- Reactivity of 3-HBA-6-hydroxylase with diethylpyrocarbonate and N-bromosuccinimide: Effect of chemical modifications on kinetic and spectral properties of the enzyme (2000), Biotechnol. Prog., 16, 577-582.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethylpyrocarbonate | 80% loss of activity after 12 min at 0.1 mM, pseudo-first-order kinetics, substrate protects, 0.1 M hydroxylamine restores 75-85% of enzyme activity | Micrococcus sp. |  |
| N-bromosuccinimide | 85-90% loss of activity at 0.04 mM, substrate protects, 10 mM N-acetylimidazole restores 85-90% of enzyme activity | Micrococcus sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 98% homogeneity | Micrococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxybenzoate + NADH + O2 | - |
Micrococcus sp. | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
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