KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADH | cosubstrate 3-hydroxybenzoate | Burkholderia cepacia | |
0.064 | - |
NADPH | cosubstrate 3-hydroxybenzoate | Burkholderia cepacia | |
0.162 | - |
3-hydroxybenzoate | cosubstrate NADH | Burkholderia cepacia | |
0.168 | - |
3-hydroxybenzoate | cosubstrate NADPH | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | 45000 | gel filtration, SDS-PAGE | Burkholderia cepacia |
44000 | - |
1 * 44000, SDS-PAGE | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.7 | - |
- |
Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + O2 | - |
Burkholderia cepacia | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
3-hydroxybenzoate + NADPH + H+ + O2 | - |
Burkholderia cepacia | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Burkholderia cepacia | |
FAD | 1 mol of FAD per mol of enzyme | Burkholderia cepacia | |
NADH | - |
Burkholderia cepacia | |
NADPH | NADH and NADPH utilized with similar efficiencies | Burkholderia cepacia |