Literature summary for 1.14.13.231 extracted from

Volkers, G.; Damas, J.M.; Palm, G.J.; Panjikar, S.; Soares, C.M.; Hinrichs, W.
Putative dioxygen-binding sites and recognition of tigecycline and minocycline in the tetracycline-degrading monooxygenase TetX (2013), Acta Crystallogr. Sect. D, 69, 1758-1767.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with tetracyclines minocycline and tigecycline at 2.18 and 2.30 A resolution, respectively. Both tetracyclines bind in a large tunnel-shaped active site in close contact to the cofactor FAD, preoriented for regioselective hydroxylation to 11alpha-hydroxytetracyclines. The bulky 9-tert-butylglycylamido substituent of tigecycline is solvent-exposed and does not interfere with TetX binding. In the TetX-minocycline complex a second binding site for a minocycline dimer is observed close to the active-site entrance. The putative dioxygen-binding cavities are located in the substrate-binding domain next to the active site	Bacteroides thetaiotaomicron

Crystallization (Comment)

Organism

in complex with tetracyclines minocycline and tigecycline at 2.18 and 2.30 A resolution, respectively. Both tetracyclines bind in a large tunnel-shaped active site in close contact to the cofactor FAD, preoriented for regioselective hydroxylation to 11alpha-hydroxytetracyclines. The bulky 9-tert-butylglycylamido substituent of tigecycline is solvent-exposed and does not interfere with TetX binding. In the TetX-minocycline complex a second binding site for a minocycline dimer is observed close to the active-site entrance. The putative dioxygen-binding cavities are located in the substrate-binding domain next to the active site

Bacteroides thetaiotaomicron

Organism

Organism	UniProt	Comment	Textmining
Bacteroides thetaiotaomicron	Q93L51	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
minocycline + NADPH + H+ + O2	-	Bacteroides thetaiotaomicron	11a-hydroxyminocycline + NADP+ + H2O	-	?
tigecycline + NADPH + H+ + O2	-	Bacteroides thetaiotaomicron	11a-hydroxytigecycline + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
TetX family tetracycline inactivation enzyme	-	Bacteroides thetaiotaomicron
TetX2	-	Bacteroides thetaiotaomicron

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Bacteroides thetaiotaomicron

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Release 2023.1 (January 2023)