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Literature summary for 1.14.13.23 extracted from

  • Chang, H.K.; Zylstra, G.J.
    Examination and expansion of the substrate range of m-hydroxybenzoate hydroxylase (2008), Biochem. Biophys. Res. Commun., 371, 149-153.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mobA, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli Comamonas testosteroni

Protein Variants

Protein Variants Comment Organism
A400G random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol Comamonas testosteroni
D416A random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol Comamonas testosteroni
H135P random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol Comamonas testosteroni
K326I random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol Comamonas testosteroni
additional information screening of random mutants from a cosmid library for altered substrate specificities Comamonas testosteroni
V257A random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol, the mutant is also active with resorcinol, hydroquinone, p-hydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3-chlorophenol, 4-chlorophenol, 4-chlororesorcinol, and 4-nitrophenol Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + NADPH + H+ + O2 Comamonas testosteroni
-
3,4-dihydroxybenzoate + NADP+ + H2O i.e. procatechuate ?
4-hydroxybenzoate + NADPH + H+ + O2 Comamonas testosteroni low activity 3,4-dihydroxybenzoate + NADP+ + H2O i.e. procatechuate ?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni Q6SSJ6 gene mobA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + NADPH + H+ + O2
-
Comamonas testosteroni 3,4-dihydroxybenzoate + NADP+ + H2O i.e. procatechuate ?
4-hydroxybenzoate + NADPH + H+ + O2 low activity Comamonas testosteroni 3,4-dihydroxybenzoate + NADP+ + H2O i.e. procatechuate ?

Synonyms

Synonyms Comment Organism
m-hydroxybenzoate hydroxylase
-
Comamonas testosteroni
MobA
-
Comamonas testosteroni