Cloned (Comment) | Organism |
---|---|
gene mobA, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli | Comamonas testosteroni |
Protein Variants | Comment | Organism |
---|---|---|
A400G | random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol | Comamonas testosteroni |
D416A | random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol | Comamonas testosteroni |
H135P | random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol | Comamonas testosteroni |
K326I | random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol | Comamonas testosteroni |
additional information | screening of random mutants from a cosmid library for altered substrate specificities | Comamonas testosteroni |
V257A | random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol, the mutant is also active with resorcinol, hydroquinone, p-hydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3-chlorophenol, 4-chlorophenol, 4-chlororesorcinol, and 4-nitrophenol | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADPH + H+ + O2 | Comamonas testosteroni | - |
3,4-dihydroxybenzoate + NADP+ + H2O | i.e. procatechuate | ? | |
4-hydroxybenzoate + NADPH + H+ + O2 | Comamonas testosteroni | low activity | 3,4-dihydroxybenzoate + NADP+ + H2O | i.e. procatechuate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | Q6SSJ6 | gene mobA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADPH + H+ + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + NADP+ + H2O | i.e. procatechuate | ? | |
4-hydroxybenzoate + NADPH + H+ + O2 | low activity | Comamonas testosteroni | 3,4-dihydroxybenzoate + NADP+ + H2O | i.e. procatechuate | ? |
Synonyms | Comment | Organism |
---|---|---|
m-hydroxybenzoate hydroxylase | - |
Comamonas testosteroni |
MobA | - |
Comamonas testosteroni |