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Literature summary for 1.14.13.220 extracted from

  • Kasai, D.; Araki, N.; Motoi, K.; Yoshikawa, S.; Iino, T.; Imai, S.; Masai, E.; Fukuda, M.
    gamma-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1 (2015), Appl. Environ. Microbiol., 81, 7656-7665.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.220

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Rhodococcus jostii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 1 * 59000, SDS-PAGE Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
resorcinol + NADH + H+ + O2 Rhodococcus jostii the enzyme gamma-resorcylate catabolic-pathway hydroxyquinol + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
resorcinol + NADH + H+ + O2 the enzyme gamma-resorcylate catabolic-pathway Rhodococcus jostii hydroxyquinol + NAD+ + H2O
-
 ?
resorcinol + NADH + H+ + O2 resorcinol i.e. benzene-1,3-diol. The enzyme has no activity with NADPH Rhodococcus jostii hydroxyquinol + NAD+ + H2O hydroxyquinol i.e. benzene-1,2,4-triol ?

Subunits

Subunits Comment Organism
monomer 1 * 59000, SDS-PAGE Rhodococcus jostii

Synonyms

Synonyms Comment Organism
tsdB
-
 Rhodococcus jostii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rhodococcus jostii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
NADH the enzyme has no activity with NADPH Rhodococcus jostii

Expression

Organism Comment Expression
Rhodococcus jostii transcription of tsdB is activated when cells are grown on gamma-resorcylate up

General Information

General Information Comment Organism
metabolism the enzyme gamma-resorcylate catabolic-pathway Rhodococcus jostii