Literature summary for 1.14.13.22 extracted from

Karande, R.; Salamanca, D.; Schmid, A.; Buehler, K.
Biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120 (2017), Biotechnol. Bioeng., 115, 312-320 .

Search for more literature for 1.14.13.22

Application

Application	Comment	Organism
synthesis	synthesis od lactones from cycloalkanes. A heterologous pathway comprising enzymes with compatible kinetics is designed in Pseudomonas taiwanensis VLB120 enabling in-vivo cascade for synthesizing lactones from cycloalkanes. The respective pathway includes cytochrome P450 monooxygenase (CHX), cyclohexanol dehydrogenase (CDH), and cyclohexanone monooxygenase (CHXON) from Acidovorax sp. CHX100. Resting cells of the recombinant host Pseudomonas taiwanensis VLB120 convert cyclohexane, cyclohexanol, and cyclohexanone to epsilon-caprolactone at 22, 80-100, and 170 U/g cell dry weight, respectively. Cyclohexane (5 mM) is completely converted with a selectivity of 65% for epsilon-caprolactone formation in 2 h without accumulation of intermediate products	Acidovorax sp. CHX100
synthesis	synthesis of lactones from cycloalkanes in Pseudomonas taiwanensis VLB120. The pathway includes cytochrome P450 monooxygenase, cyclohexanol dehydrogenase, and cyclohexanone monooxygenase from Acidovorax sp. CHX100. Recombinant P. taiwanensis converts cyclohexane, cyclohexanol, and cyclohexanone to epsilon-caprolactone at 22, 80-100, and 170 U/gCDW, respectively. Cyclohexane (5 mM) is completely converted with a selectivity of 65% for epsilon-caprolactone formation in 2 hr without accumulation of intermediate products. Analogous lactones can be obtained from cyclooctane and cyclodecane	Acidovorax sp. CHX100

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pseudomonas taiwanensis VLB120	Acidovorax sp. CHX100

Organism

Organism	UniProt	Comment	Textmining
Acidovorax sp. CHX100	A0A2D0WG32	-	-

Synonyms

Synonyms	Comment	Organism
CHXON	-	Acidovorax sp. CHX100

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Release 2023.1 (January 2023)