Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | competitive against NADPH | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
NCIB 9871 | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Acinetobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | reaction mechanism | Acinetobacter sp. | |
cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | kinetic studies | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexanone + NADPH + O2 | activity limited to cyclic ketones | Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? |
Synonyms | Comment | Organism |
---|---|---|
CHMO | - |
Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Acinetobacter sp. | |
NADPH | - |
Acinetobacter sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NADP+ | pH 9.0, 25°C | Acinetobacter sp. |