KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Nocardia globerula | |
0.0185 | - |
NADPH | isozyme type 1 | Nocardia globerula | |
0.02 | - |
NADPH | isozyme type 2 | Nocardia globerula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Nocardia globerula | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia globerula | - |
enzyme exists in 2 electrophoretically distinct isoforms: type 1 and type 2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxycyclohexanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O | - |
? | |
2-methylcyclohexanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
cyclobutanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
cycloheptanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
cyclohexanone + NADPH + O2 | - |
Nocardia globerula | 6-hexanolide + NADP+ + H2O | - |
? | |
cyclooctanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
cyclopentanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000-59000, SDS-PAGE | Nocardia globerula |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
2 min, 40% loss of activity for isozyme type 1, 7% loss of activity for isozyme type 2 | Nocardia globerula |
45 | - |
1 min, 40% loss of activity for isozyme type 1, 18% loss of activity for isozyme type 2 | Nocardia globerula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.1 | broad, isozyme type 2 | Nocardia globerula |
8 | - |
sharp, isozyme type 1 | Nocardia globerula |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | pH 6.0: about 40% of activity maximum with isozymes type 1 and type 2, pH 10.5: about 10% of activity maximum with isozyme type 1, about 60% of activity maximum with isozyme type 2 | Nocardia globerula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 0.62 mol of FAD per mol of holoenzyme type 1, 0.75 mol of FAD per mol of holoenzyme type 2 | Nocardia globerula | |
NADPH | absolutely specific for NADPH as electron donor | Nocardia globerula |