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Literature summary for 1.14.13.210 extracted from

  • Haigler, B.E.; Suen, W.C.; Spain, J.C.
    Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT (1996), J. Bacteriol., 178, 6019-6024.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dntB, sequence comparisons Burkholderia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60200
-
1 * 60200, SDS-PAGE Burkholderia sp.
65000
-
gel filtration Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp. P71029 gene dntB
-
Burkholderia sp. RASC P71029 gene dntB
-

Purification (Commentary)

Purification (Comment) Organism
native enzme to homogeneity from strain DNT by anion exchange chromatography and gel filtration Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methyl-5-nitrocatechol + O2 + NADPH + H+ the enzyme is highly specific for 4-methyl-5-nitrocatechol Burkholderia sp. 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
?
4-methyl-5-nitrocatechol + O2 + NADPH + H+ the enzyme is highly specific for 4-methyl-5-nitrocatechol Burkholderia sp. RASC 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+
-
?
4-nitrophenol + O2 + NADPH + H+ 58% of the activity with 4-methyl-5-nitrocatechol Burkholderia sp. 4-benzoquinone + nitrite + H2O + NADP+
-
?
4-nitrophenol + O2 + NADPH + H+ 58% of the activity with 4-methyl-5-nitrocatechol Burkholderia sp. RASC 4-benzoquinone + nitrite + H2O + NADP+
-
?
additional information the enzyme has a narrow substrate specificity. 3-Methyl-4-nitrophenol, 3-methyl-4-nitrocatechol, 4-nitrophenol, 3-nitrophenol, and 4-chlorocatechol are no substrates Burkholderia sp. ?
-
?
additional information the enzyme has a narrow substrate specificity. 3-Methyl-4-nitrophenol, 3-methyl-4-nitrocatechol, 4-nitrophenol, 3-nitrophenol, and 4-chlorocatechol are no substrates Burkholderia sp. RASC ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60200, SDS-PAGE Burkholderia sp.

Synonyms

Synonyms Comment Organism
4-methyl-5-nitrocatechol oxygenase
-
Burkholderia sp.
dntB
-
Burkholderia sp.
MNC
-
Burkholderia sp.
MNC monooxygenase
-
Burkholderia sp.

Cofactor

Cofactor Comment Organism Structure
FAD the purified enzyme contains 1 mol of FAD per mol of protein Burkholderia sp.
NADH the enzyme uses either NADH or NADPH as electron donors, and NADPH is the preferred cofactor Burkholderia sp.
NADPH the enzyme uses either NADH or NADPH as electron donors, and NADPH is the preferred cofactor Burkholderia sp.

General Information

General Information Comment Organism
evolution enzyme DntB contains the highly conserved ADP and flavin adenine dinucleotide (FAD) binding motifs characteristic of flavoprotein hydroxylases Burkholderia sp.