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Literature summary for 1.14.13.208 extracted from

  • Zaar, A.; Gescher, J.; Eisenreich, W.; Bacher, A.; Fuchs, G.
    New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii (2004), Mol. Microbiol., 54, 223-238.
    View publication on PubMed
Search for more literature for 1.14.13.208

Activating Compound

Activating Compound Comment Organism Structure
BoxC addition of the putative ring cleaving enzyme BoxC leads to a several-fold acceleration of the initial rate and completes conversion of benzoyl-CoA. BoxC might facilitate the binding of BoxA to BoxB and thus lead to the observed rate increase Aromatoleum evansii

Inhibitors

Inhibitors Comment Organism Structure
KCl higher salt concentration (500 mM KCl) results in sixfold lower activity suggesting that the interaction of the protein components is affected by high salt concentration Aromatoleum evansii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 benzoyl-CoA pH 8, 22°C, addition of the putative ring cleaving enzyme BoxC leads to a several-fold acceleration of the initial rate and completes conversion of benzoyl-CoA. Because of this complex behaviour of the oxygenase system, the apparent Km value for benzoyl-CoA can only be estimated from the time curve of benzoyl-CoA concentration in an assay mixture that contains BoxAB and the putative ring-cleaving enzyme BoxC. This curve shows a half-maximal rate at a benzoyl-CoA concentration of 0.03 mM Aromatoleum evansii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein, which acts as reductase. BoxB is a monomeric iron-protein. Subunit BoxB contains 1.6 mol of iron per mol of protein, addition of 1 mM Fe2+ to the assays has no stimulatory or inhibitory effect on the catalytic turnover Aromatoleum evansii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 2 * 46000 (BoxA) + 50000 (BoxB), boxA/boxB system, BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein, which acts as reductase. BoxB is a monomeric iron-protein (50000-60000 Da, gel filtration. 50000 Da, SDS-PAGE) Aromatoleum evansii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii KB740 the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Aromatoleum evansii Q9AIX6 and Q9AIX7 Q9AIX6: subunit A, Q9AIX7: subunit B
-
Aromatoleum evansii KB740 Q9AIX6 and Q9AIX7 Q9AIX6: subunit A, Q9AIX7: subunit B
-

Purification (Commentary)

Purification (Comment) Organism
His6-tagged component B of benzoyl-CoA oxygenase, component A of benzoyl-CoA oxygenase Aromatoleum evansii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-fluorobenzoyl-CoA + NADPH + H+ + O2 83% of the activity with benzoyl-CoA Aromatoleum evansii ?
-
 ?
2-fluorobenzoyl-CoA + NADPH + H+ + O2 83% of the activity with benzoyl-CoA Aromatoleum evansii KB740 ?
-
 ?
4-fluorobenzoyl-CoA + NADPH + H+ + O2 30% of the activity with benzoyl-CoA Aromatoleum evansii ?
-
 ?
4-fluorobenzoyl-CoA + NADPH + H+ + O2 30% of the activity with benzoyl-CoA Aromatoleum evansii KB740 ?
-
 ?
benzoyl-CoA + NADPH + H+ + O2 the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii Aromatoleum evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
benzoyl-CoA + NADPH + H+ + O2 no activity with NADH as electron donor Aromatoleum evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
benzoyl-CoA + NADPH + H+ + O2 the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii Aromatoleum evansii KB740 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
benzoyl-CoA + NADPH + H+ + O2 no activity with NADH as electron donor Aromatoleum evansii KB740 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
additional information no activity with benzoate or phenylacetyl-CoA Aromatoleum evansii ?
-
 ?
additional information no activity with benzoate or phenylacetyl-CoA Aromatoleum evansii KB740 ?
-
 ?

Subunits

Subunits Comment Organism
? 2 * 46000 (BoxA) + 50000 (BoxB), boxA/boxB system, BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein, which acts as reductase. BoxB is a monomeric iron-protein (50000-60000 Da, gel filtration. 50000 Da, SDS-PAGE) Aromatoleum evansii

Synonyms

Synonyms Comment Organism
benzoyl-CoA dioxygenase/reductase
-
 Aromatoleum evansii
BoxA/BoxB system
-
 Aromatoleum evansii
BoxBA
-
 Aromatoleum evansii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Aromatoleum evansii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Aromatoleum evansii

Cofactor

Cofactor Comment Organism Structure
NADPH no activity with NADH Aromatoleum evansii

pI Value

Organism Comment pI Value Maximum pI Value
Aromatoleum evansii calculated from sequence
-
 5.6